MICKOWGIOGRAPHY WITH PROJECTION MICROSCOPE 



graphic and Autoradiographic Researches 

 on Hard Dental Tissues," Ada Anat., 28, 

 217-258, 1956. 



121. Amprino, R., Engstrom, A., "Studies on 



X-ray Absorption and Diffraction of bone 

 tissue," Acta Anat., 17, 1-22,-1952. 



122. Belanger, L., "Microradiography with a 



Polonium Alpha Source," J. Bioph. & Bio- 

 chem. Cijtology, 6, 197-202, 1959. 



123. Bellman, S., Exgfeldt, B., "Kidney Le- 



isons in Experimental Hypervitaminosis 

 D," A>7ier. J. Bad., 74, 288-294, 1954. 



124. Bergandall, G., Exgfeldt, B., Preparing 



Material for ^Microradiography, "Acta 

 Pathol. & Microbiol. Scandinavica," 49, 

 30-38, 1960. 



125. Bohatirchuk, F., "Uber Ergebnisse der Mik- 



rorontgenographie," Acta Rad., 27, 351- 

 365, 1953. 



126. Bohatirchuk, F., Microradiographical Data 



on Aging Bone Atrophy as Seen in Micro- 

 radiographs and Colored Specimens," J. of 

 Gerontology, 15, 142-149, 1960. 



127. Bohatirchuk, F., "Reversibility of Bone 



Changes in Physiological Aging," Report 

 to the IVth International Gerontological 

 Congress, Merano, Venice, 1957. Tito Mat- 

 tioli, Fidenza. 



128. Bohatirchuk, F., "Experimental Bone 



Atrophy in Rabbits in Comparison with 

 Bone Atrophy in Aging Humans as Seen in 

 Historadiographs and Colored Specimens," 

 Report to the Vth Int. Gerontological Con- 

 gress, San Francisco, 1960 (in press). 



129. Brookes, M., "The Vascular Architecture of 



Tubular Bone in the Rat," Anat. Rec, 132, 

 25-47, 1958. 



130. Brookes, M., "The Vascular Reaction of 



Tubular Bone to Ischaemia in Peripheral 

 Occlusive Vascular Disease," /. Bone & 

 Joint Surg. (Brit.), 42b, 110-125, 1960. 



131. Carreto, L., "Contributo alio Studio della 



Matrice Ossea Neodeposta," Archive 

 "Putti," X, 211-230, 1958. 



132. CossLETT, V. E., "Microscopy with X-rays," 



Nature, 183 1423-1427, 1959. 



133. Grampp, W., Hallen, O., "Microphotometry 



in X-raj' Contact Microscopj%" Exp. Cell 

 Research, 19, 83-92, 1960. 



134. Grampp, W., Hallen, O., Rosenbren, B., 



"Mass Determination by Interference and 

 X-raj' Microscopy," Exp. Cell Research, 19, 

 437-442, 1960. 



135. Istock, J. T., Miller, C. W., Chambers, 



F. W., Lyon, H. W., "Historadiography of 

 Hard and Soft Tissues, U.S.A. Armed 

 Forces Med. J., 11, 497-506, 1960. 



136. Lagergren, C, Lindbom, A., Sodergerg, 



G., "Hypervascularization in Chronic 

 Inflammation Demonstrated by Angiog- 

 raphy," Acta Radiol., 49, 441-452, 1958. 



137. Lagergren, C, Lindbom, A., Sodergerg, 



G., "Vascularization of Fibromatous and 

 Fibrosarcomatous Tumours," Acta Radiol., 

 53, 1-16, 1960. 



138. Ong Sing Poen, "Microprojection with X- 



rays," Martinus Nijhoff, The Hague, The 

 Netherlands, 1959. 



139. PuGH, M. N., Savchuk, W. B., "Suggestions 



on the Preparation of Undecalcified Bone 

 for Microradiography," Stain. Techn., 33, 

 287-295, 1958. 



140. Rosengren, B. H. O., "Determination of 



Cell Mass by Direct X-ray Absorption," 

 Acta Radiol. Siipplementu , 178, 1959. 



141. Scott, B. L., Pease, D. C, "Electron Micros- 



copy of Epiphysial Apparatus," Anat. 

 Rec, 126, 465-480, 1960. 



142. Strandh, J., "Microchemical Studies on Sin- 



gle Haversian System," Exp. Cell Research, 

 19, 515-530, 1960. 



143. Trueta, J., Morgan, J. D., "The Vascular 



Contribution to Osteogenesis," /. Bone & 

 Joint Surg., Brit., 42b, 97-109, 1960. 



144. Vincent, J., "Correlation Entre la Microra- 



diographie et ITmage en Lumiere Polaris^e 

 de I'Os Secondaire," Exp. Cell Research, 12, 

 422-424, 1957. 



145. Vincent, J., "Etude Microradiographique de 



rOssification Enchondrale," Acta Anat., 

 40, 121-129, 1960. 



F. Bohatirchuk 



MICROANGIOGRAPHY WITH THE 

 PROJECTION MICROSCOPE 



The use of x-rays for microscopy derives 

 from the fact that x-rays have a shorter 

 wavelength than Hght and therefore a 

 greater penetration and higher resolving 

 power. Contact microradiography (1, 2) has 

 long been available to biologists, but it is 

 only recently that the principles of reflection 

 (3) and projection (4) have been applied to 

 x-ray microscopy. The first x-ray projection 

 microscope was reported in 1952 by Cosslett 

 and Nixon. 



The projection method of x-ray micros- 

 copy rests on the fact that a point source of 

 x-rays casts an enlarged image of a nearby 



627 



