24 



Cleavage of Benzoylalanine and Acetylglycine 



[Oct. 



presence of the glycine group or the presence of an aromatic acid 

 radical? Or does hippuric acid simply represent a type of substi- 

 tuted amino acids, all of which are hydrolyzed by the same enzyme? 



With the view of throwing some light on this question, ben- 

 zoylalanine and acetylglycine were subjected to the action of en- 

 zyme preparations capable of hydrolyzing hippuric acid. Benzoyl- 

 alanine may be regarded simply as a homologue of hippuric acid. 

 Neither this substance nor acetylglycine, as far as \ve are aware, 

 occurs in nature nor does either result from any known biological 

 process. 



Mold cultures were made as previously described^ and the press 

 Juice allowed to act upon the sodium salt of benzoylalanine and 

 acetylglycine respectively. Since the experiments were conducted 

 exactly as outlined in our previous paper, the details will not be re- 

 peated here. The formol titration was made after the enzyme and 

 Substrate had been in contact for two weeks at room temperature. 

 The results are given in the accompanying tables. 



TABLE I 



Data on the cleavage of hensoylalanine 



TABLE 2 



Data on the cleavage of acetylglycine 



3 Dox and Neidig: Loc. cit. 



