I9I3] I- K. Phelps Sy 



alcoholic soda extract of P. glaiicuni were found oleic and palmitic 

 acids, a fatty acid melting at 54° C, a fatty acid, which appears to 

 be elaidic acid, hypoxanthin, guanin, adenin, histidin, thymin and 

 cholin. In the direct alcohol extract were found mannite, choles- 

 terols, hypoxanthin, and cerebrosides. From mold grown on 

 RauHn's Solution plus peptone a small amount of guanidin was ob- 

 tained. In the culture Solution, after a number of weeks' growth, 

 were found fatty acids, purin bases, a small quantity of a histidin- 

 like substance, pentose sugar, unidentified aldehydes, etc. Many of 

 these Compounds have been found in soil and the conclusion is drawn 

 that microorganisms, such as yeasts, bacteria and molds, play an 

 important part in their formation. 



A method for the determination of small amounts of fat. 

 W. R. Bloor. {Laboratories of Chemistry of Queens University, 

 Kingston, Canada, and of Biological Chemistry of Washington 

 University, St. Louis, Mo.) The method consists essentially in 

 extracting the fat from the tissue or liquid with an excess of alcohol- 

 ether (25 percent ether), measuring an aliquot portion of the 

 filtered extract into distilled water and determining the amount of 

 fat by comparison of the cloudy Suspension so obtained with a 

 Standard fat Solution by the use of the nephelometer. The method 

 has given good results with blood and milk. 



Nitrogenous hydrolytic products of several Phosphatids. C. 

 G. MacArthur and G. Norbury. (University of Illinois.) 

 Sheep-brain kephalin, sheep-brain lecithin, ox-heart cuorin and ox- 

 heart lecithin were prepared, purified, and then hydrolyzed in a 

 dilute hydrochloric acid Solution. In each case the fatty acid residue 

 contained nitrogen, usually about one-sixth of the total. The fil- 

 trate nitrogen was separated by a special method into four fractions, 

 representing (i) ammonia, (2) chohn or other basic Compounds, 

 (3) amino acids, or Compounds not precipitated by platinum chloride 

 but precipitated by mercuric acetate in a sodium carbonate Solution, 

 and (4) the filtrate from (3). The two lecithins contain about two- 

 fifths of the nitrogen in form 2, while kephalin and cuorin contain 

 practically none. In all of them, fraction 3 is large, varying from 

 one-third to one-half. 



Fatty acids from kephalin. L. V. Burton and C. G. Mac- 



