Outline of methods for the Isolation 



' Soluble in water 

 (reject) 



r Precipitate (4) 



' (o) Acidify three- 

 fourths of 

 extract with 

 HNO3 



I. 



31 



13 



Soluble in alco- 

 hol 



Insoluble in water 



14 



Acid filtrate(is). 

 Extract with 

 ether (14) 



Insoluble in alco- 

 hol (reject) 



Ether extract contains. 



16 



r(a) 



15 



21 



Neutralized 

 aqueous Solu- 

 tion 



32 



(&) Acidify one- 

 fourth of 

 extract with 

 H,S04 .... 



C Precipitate ; 

 bine with 



25 



33 



com- 

 3 



Precipitated 

 by Pb ace- 

 tate 



(b) 



Precipitated 

 by AgNOs 



(c) 



Precipitated 

 in alkaline 

 sol. with 

 Fehling 

 sol. and 

 glucose , . 



Acid filtrate. Pre- 

 cipitate with 

 phosphotungstic 

 acid (34) 



34 



Filtrate (reject) 



Precipitate. De- 35 

 compose with 

 Ba(OH)o, etc. 



37 



fFiltrate. Add 

 solid Ba(0H)2 



Precipitate 

 tains . . . 



con- 



218 



