304 Proceedings Columbia Biochemical Association [Jan. 



procedures were employed with the phenols under consideration. 

 All, except tyrosin, showed oxygen absorption or rather, combina- 

 tion with oxygen. Curves having the time element for abscissae 

 and heights of liquid in tube for ordinates, brought out the interest- 

 ing fact that the rate of oxygen absorption, or the rate of intensity 

 of color formation, bears a direct relationship to the number of OH 

 groups in the phenol. The work is now being continued along the 

 following lines : The application of the foregoing methods to all 

 obtainable Compounds containing the phenolic grouping; the colori- 

 metric measurement of the rate of color formation; the spectro- 

 scopic behavior of the different colored products; and the isolation 

 and examination of the pigments. 



This research, which is being carried out under Professor Gies' 

 direction, may have an important bearing on plant pigments; and 

 it is in connection with work on the latter, in which Professor Gies 

 and the author are engaged, that it has been undertaken. 



110. A clinical method for the quantitative estimation of 

 sugar in small amounts of blood. Max Kahn. {Washington, 

 D. C.) The test recommended is based on Bang's^ process. Con- 

 trol tests were made with various known Solutions of glucose and 

 accurate quantitative results were obtained. The process is very 

 simple and should require not more than ten to fifteen minutes for 

 the whole analysis. 



Two Solutions are prepared in the following manner: (a). Cop- 

 per Solution. Dissolve 500 gm. of potassium carbonate, 400 gm. 

 of potassium sulfocyanate and 100 gm. of potassium bicarbonate 

 in 1200 c.c. of water, warming if necessary to 50-60° C, and then 

 cooling to room temperature. Add 150 c.c. of an aqueous Solution 

 of 25 gm. of copper sulfate (crystallized). The sol. is then made 

 up to 2000 c.c; it keeps indefinitely. {b). Hydroxylamine Solu- 

 tion. Dissolve 200 gm. of potassium sulfocyanate in 1500 c.c. of 

 water and add a sol. of 6.55 gm. of hydroxylamine sulfate in water. 

 Make up with water to 2000 c.c. This sol. also keeps well in a 

 dark colored bottle. Equal volumes of these two Solutions should 

 exactly correspond to each other, The presence of proteins does 

 not interfere with this test. 



6 Bang: Zeit. f. physiol. Chem., 1909, Ixiii, p. 443; ibid., 1910, Ixv, p. 497; 

 Biochem. Zeit., 1908, vii, p. 327; ibid., 1909, xi, p. 538; Hammarsten-Festschrift, 

 1906, No. 2. 



