I9I4] Alfred P. Lothrop 2,07 



112. Urinary catalase in health and disease. Max Kahn 

 AND Charles J. Brim. {Beth Israel Hospital Chemical Lahora- 

 tory.) Traces of catalase were found in normal urine that had 

 been freshly voided. In the urines of 75 patients suffering with 

 various ailments, the catalase content varied. It was found especially 

 high in urines that reacted positively f or blood, bile or acetone. Quite 

 a number of urines of patients suffering from Cancer were tested for 

 catalase, and no Variation from the normal was observed, except in 

 those cases where the liver was involved and the urine contained 

 biliary pigments, or where the disease was very advanced and the 

 urine showed the presence of acetone. Four cases of lympho- 

 sarcoma and Hodgkin's disease gave very high catalase content in 

 the urine. In severe diabetes, patients with acidosis eliminated very 

 largely increased amounts of catalase in the urine; on the other 

 hand, diabetics who had no acetonuria showed no Variation in the 

 amount of urinary catalase. Typhoid fever patients and others 

 with general septic infections excreted considerable catalase in the 

 urine. 



113. Studies on the Classification of the colon group. I. J. 

 Kligler. (Department of Public Health, American Mtiseum of 

 Natural History.) Eighty organisms, generally classed under the 

 Colon group, were subjected to a series of fermentative and other 

 tests with a view of determining their natural grouping as based 

 on biometric principles. The following tests were employed: (i) 

 Morphology, gram; (2) fermentation of glucose, lactose, sucrose, 

 rafifinose, glycerol, mannite, dulcite, salicin and inulin; (3) coagu- 

 lation of milk; (4) liquefaction of gelatin; (5) productionof indol; 

 (6) reduction of nitrate; (7) V and P reaction. Fifty-seven of the 

 strains feil into the lactose- fermenting division; twenty did not 

 ferment lactose, but fermented glucose; three failed to ferment 

 sugars. 



Acid production, as determined by titrating aliquot portions of 

 the broth with phenolthalein as an indicator, was found to be a more 

 constant and a more reliable differential test than gas production, 

 as ordinarily determined. The degree of initial acidity had no ap- 

 preciable effect on the final acidity, which was quite constant, reach- 

 ing its maximum on about the fourth day. 



The fifty-seven lactose- fermenters attacked mannite, glycerol. 



