I9I4] 



/. Bronfenbrenner and J. Rocktnan 



383 



Seen from the following: Tuberculin IV was precipitated with 

 glacial acetic acid; the precipitate was extracted repeatedly with 

 alcohol-ether mixture and the extract evaporated to dryness; the 

 residue was taken up with 2 cc. of methyl alcohol and subsequently 

 further diluted with physiological salt sol. to the volume of the 

 tubercuHn from which the lipins originated. 



TABLE I 



Data pertaining to differences among various preparations of Besredka tuberculin 



This emulsion of lipins was then used asif it weresyphiliticanti- 

 gen in parallel series with a preparation of lipins originating from 

 beef heart, which we are using for the routine diagnosis of syphilis. 

 Both emulsions were tested in varying quantities against a constant 

 amount of complement, amboceptor and known syphilitic serum 

 (Table 2). 



TABLE 3 



Comparative data an lipin products from Besredka tuberculin as syphilitic 



antigens 



Syphilitic antigen 



Lipins from Tuberculin IV 

 Lipins from Tuberculin II. 



O.I 



cc. 



+ 

 + 



0.07 

 cc 



+ 

 + 



o.os 

 cc. 



+ 

 < + 



0.03 



cc 



+ 



0.02 

 cc. 



+ 



o.oi 

 cc. 



+ 



0.007 

 cc. 



< + 



o.oos 

 cc. 



<< + 



0.003 

 cc. 



