I9I4] Alfred P. Lothrop 4^7 



bers of the pyrimidin group, and he therefore concluded that the new 

 Compound must contain a pyrimidin ring. 



Funk's results were obtained with 54 kg. of rice polishings. 

 Since it was not practicable to work up such a large quantity, 2 kg. 

 were used in a prehminary exp. These were extracted, for 4 days, 

 with 8 Hters of 0.5 percent sol. of hydrochloric acid in alcohol. The 

 filtered extract was conc. in vacuo at 37-50° C. The conc. syrup, di- 

 luted with water, was neutraHzed with barium hydroxid, ehminating 

 the phytin at once and f orming soaps with the f ats. The heavy pre- 

 cipitate was filtered off and sulfuric acid to approximately 5 percent 

 added to the filtrate. To this, phosphotungstic acid was added 

 in excess, the precipitate was filtered off on a Büchner funnel, 

 washed and decomposed with excess of barium hydroxid by grind- 

 ing with water in a mortar. A distinct ammoniacal odor was noted, 

 indicating decomposition of amino Compounds. The mass was then 

 filtered, the filtrate freed from excess of barium with carbon dioxid 

 and evaporated to a small volume, from which the remainder of the 

 bariiim was removed with dilute sulfuric acid. 



The conc. filtrate, containing the merest trace of acid, cured 

 chickens inside of 4-6 hr. when injected into the pectoral muscles. 

 The quantity of substance was small, and the eures by no means 

 uniformly obtained, but two experiments with chickens in violent 

 convulsions demonstrated beyond a doubt that some curative sub- 

 stance must have been present. 



An attempt was made, by further treatment of the extract from 

 12 kg. with the silver nitrate-barium hydroxid method, to obtain 

 even a minute quantity of pure vitamin, but without success. 



129. The appearance of non-colloidal, ninhydrin-reacting 

 substances in the urine under normal and pathological condi- 

 tions, and during pregnancy. F. H. Falls and Wm. H. 

 Welker. {Lab. of Med. Research and Physiol. Chem., Coli, of 

 Med., Univ. of III., Chicago, III.) In this investigation 124 urines 

 from normal, pathological, and pregnant cases, were studied with 

 respect to their reaction with ninhydrin. For the removal of col- 

 loidal substances an excess of aluminium hydroxid cream was used 

 and the ninhydrin test was applied to the filtrate. A series of urines 

 were also studied in which the ninhydrin test was applied directly to 



