114 Indiatwpolis Biochemical Meeting: Ahstracts [Sept 



able receptacle Standing on the floor under the front end of the 

 cage. The urine passes through this hole into a shallow pan 

 suspended from the hopper trough, immediately beneath. This 

 pan has an elongated spoiit, leading forward, through which the 

 urine flows into another receptacle Standing on the floor beside the 

 one provided for the düng. 



The cage is simple in construction. It was made by local car- 

 penters with the aid of a tinsmith, at a cost of thirty-seven doUars. 

 The complete cage occupies a floor space of about two by four feet, 

 is seven feet high and can be easily carried by two men. The 

 cage is equally applicable to studies on sheep. 



On the Lipins of the Heart Muscle of the Ox 



JACOB ROSENBLOOM 



(Laboratory of Biological Chemistry of Columbia University, at 

 ^ the College of Physicians and Surgeons, New York.) 



MacLean and Williams^ have found that the essential fat of the 

 liver has the properties of phospholipin. They think it probable that 

 the fatty matter from certain other organs is of the same nature. 

 They find by extraction of the liver with ether and alcohol, at room 

 temperature, that 84 per cent. of the total extract is phospholipin in 

 quality, whereas, if the extraction is carried out at the temperature 

 of the boiling solvent, only about 40 per cent. of the extract partakes 

 of the properties of phospholipin. MacLean and Williams believe 

 that such treatment with the boiling solvent causes a cleavage of 

 the tissue phospholipin, with a consequent increase in the amount of 

 neutral fat in the extract. 



In a study of the lipins of the heart muscle of the ox, practically 

 identical percentages of neutral fat and phospholipin were found 

 by the writer in the ether and alcohol extracts which had been 

 obtained by treatment with the respective solvents at room tempera- 

 ture and also at their boiling temperatures. It is possible, however, 

 that the ether and alcohol extracts of the liver contain substances of 

 a lipin nature which are more easily decomposed than those in 

 similar extracts of heart muscle. 



^ MacLean and Williams : Biochemical Journal, 1909, iv, p. 455. 



