191 1] Carl L. Aisher g 123 



Methods o£ Estimating Moisture in Tissues 



WALDEMAR KOCH 



(Laboratory of Pharmacology, Chicago University.) 



With valuable biological material it is sometimes desirable to 

 make water estimations and the estimations of the other constituents 

 on the same sample. As there is danger of decomposing the con- 

 stituents by the high temperature employed for drying in the official 

 method, a comparison of this method with the one devised some 

 years ago^ and used in this laboratory was made. The results are 

 recorded in the following table. 



W.8. W. 21. 



Direct with Alcohol. Dried by Heat at 95° C. 



Proteins 48.5 47.5 



Phosphatids 21.6 16.3 



Cerebrosids 8.8 9.4* 



Sulphatids 2>-6 4-3* 



Undetermined lipoids 8.2 li.o* 



Organic and inorganic extractives 9.3 11.6 



lOO.O lOO.I 



Lipoid P in per cent. of total P. . . 62.5 53.6 



The Preparation of Tissue for Toxicological Examination 



JAMES P. ATKINSON 



{Chemical Laboratory of the Department of Health, 



New York City.) 



The finely minced tissue is digested with artificial gastric Juice. 

 The Solution is filtered and extracted for alkaloids in the usual 

 way. After this extraction the material is evaporated with nitric 

 acid and then examined for metallic poisons. This method has 

 three advantages : ( i ) The examination may be completed within 

 three days, (2) less personal attention is required, and (3) the 

 tissue is completely broken down and there fore allows a better 



^ Koch : Journal of the American Chemical Society, 1909, xxxi, p. 1335. 



' Variation due to difiference of age. 



• Increase due to f atty acids f rom destruction of Phosphatids. 



