191 1] Max Kahn 239 



could ooze out and vitiate the analytlc results. The liver was next 

 to the last organ to be taken out. The heart was the last to be re- 

 moved. In each case the heart was cut open and quickly flushed 

 with distilled water. Before the body was opened, and while the 

 parts were being removed, special care was taken to prevent acci- 

 dental introduction of foreign matter. 



III. FIRST EXPERIMENT: DURATION, TWO MONTHS 



The first experiment was started on June 29, 191 1. The dog 

 (male) weighed 8.66 kilos. He was given the following mixed diet : 

 Meat,^ 85 grams; aluminized biscuit, 128 grams; lard, 25.5 grams; 

 bone ash, 8.5 grams; water, 298 c.c. The dog gained weight on 

 this diet. 



On (and after) July 2y, when the animal weighed 9.65 kilos, 

 the lard was omitted and the same weight of meat substituted. 



On August 29, when the dog weighed 10.72 kilos, and the ex- 

 periment had been in progress for two months, the animal was 

 killed. 



The dog was bled to death from the left femoral artery under 

 Cocain anesthesia. The blood was defibrinated as it accumulated. 

 About 510 grams of blood were removed. No more could be drawn 

 from this artery. The heart beat was strong, and the respiration deep 

 and vigorous. The animal seemed to stand well the loss of blood 

 and gave no evidence of distress. The dog was allowed to lie on the 

 board for one half hour. Cocain was then injected over the region 

 of the right anterior iliac artery, and 155 grams more of blood were 

 withdrawn. The blood clotted very slowly. The bleeding was very 

 thorough. No free blood was noticed in any of the removed organs 

 except the heart. In the heart the quantity of residual blood was 

 very slight. 



The total amount of blood removed was 665 grams. Of this 

 quantity, 210 grams were oxidized, made up to 500 c.c. and 50 c.c. 

 portions of the liquid analyzed for aluminium. The total volume of 

 blood (655 grams) contained the aluminium in 10.9 mg. of AlgOg. 



The results of the analysis of the tissues for aluminium are 

 summarized in Table i. 



• Several samples of meat were analyzed for aluminium. None was found. 



