240 ■Aluminium from Aluminized Foods [Dec 



TABLE I. 



Amounts of AhOa obtained from the tissues of the dog in the first experiment 



Amount of AloOs 

 Tissue Weight Total Per loo Grams 



Grams mg. mg. 



Bile HO 141 130 



Blood 655 10.9 1.7 



Bone (femur) 48 0.8 1.6 



Hone (skull) 62 None — 



Brain 68 None — 



Heart 75 None — 



Kidneys 70 4.2 6.0 



Liver 179 6.4 3.6 



Muscle 100 3.2 3.2 



Pancreas 39 1-9 5-0 



Spleen 21 1.7 8.0 



In Order to get rid of precipitated calcium sulfate in the analysis 

 of bone, the clear supernatant fluid was decanted after the addition 

 of sulfuric acid. In this process sHght loss of aluminium may have 

 occurred. 



IV. SECOND EXPERIMENT : DURATION. 55 DAYS 



A small female dog, weighing 5.68 kilos on June 29, was given 

 the following daily diet: Meat, 57 grams; aluminized biscuit, 85.5 

 grams; lard, 17.0 grams; bone ash, 5.5 grams; water, 200 c.c. 



On (and after) July 27, when the dog weighed 6.45 kilos, lard 

 was omitted from the food but the same weight of meat was sub- 

 stituted. 



On August 23, 55 days after the beginning of the experiment, 

 the dog was killed. It weighed 6.64 kilos. 



The dog was bled to death from both femoral arteries simul- 

 taneously under local cocain anesthesia. The dog was almost per- 

 fectly exsanguinated. Upon opening the abdomen the tissues were 

 Seen to be very pale, and no points of bleeding were noticed. The 

 Organs enumerated in Table 2 were removed. The liver was next 

 to the last to be taken out. A very slight contamination of the liver 

 with bile was unavoidable in this case.^^ The heart was the last 

 organ to be removed from the body. 



"Very great care was exercised in the removal of Organs. Rubber gloves 

 were used and after each organ was removed, the gloved hands were washed in a 

 running stream of distilled water. The same precaution was taken with the 

 instruments. 



