V 



iQii] Max Kahn 243 



VI. ELIMINATION OF ALUMINIUM IN THE URINE 



Although it was not the purpose of these experiments to deter- 

 mine the quantitative excretion of aluminium in the urine, we con- 

 cluded, nevertheless, to collect some urine and analyze it for alu- 

 minium. 



Special precautions were taken in the collection of this urine, A' 

 fourth dog, a female weighing 13.85 kilos on June 29, was fed daily 

 thereafter for nearly three months on the following diet : Meat, 140 

 grams; aluminized biscuit, 210 grams; lard, 42 grams; bone ash, 

 14 grams ; water, 490 c.c. 



The dog gained steadily in weight. On September 13, the 

 special urine collections were started. It was obvious that under 

 the dietary conditions of these experiments we could not collect 

 urine in the receiver under the cage, because in this way the urine 

 would surely be contaminated by feces containing aluminium. 

 Catheterization was avoided for fear of setting up a cystitis. Prof. 

 Gies suggested the expedient of occasionally taking the dog out of 

 the cage, allowing her under control to walk about the laboratory, 

 and collecting urine in a clean porcelain dish as it was passed. In 

 this way 610 c.c. of uncontaminated urine were collected during a 

 period of about ten days (Sept. 13-23). All other fractions of the 

 urine were rejected. 



The uncontaminated urine was divided into two equal portions. 

 Each portion was evaporated to a small bulk, then oxidized with nitric 

 acid and sulfuric acid, and the aluminium determined, with the fol- 

 lowing results : AI0O3 in part A, 6.2 mg; AI2O3 in part B, 5.5 mg. ; 

 average amount of AI2O3 per 100 c.c, 1.8 mg. 



VIT. GENERAL CONCLUSIONS 



1. When biscuits baked with alum baking powder are fed in a 

 mixed diet to dogs, aluminium passes in considerable amounts into 

 the blood. 



2. Such absorbed aluminium circulates freely and, although it 

 does not show a tendency to increase proportionately in the blood, it 

 accumulates to some extent in various parts of the body. The bile 

 contains a particularly large amount of aluminium under such cir- 

 cumstances. The pancreas, spieen, liver, muscle and kidneys con- 



