48o 



Enzymes as Factors in Edema 



[Mar. 



and the elimination, of diffusible colloid cleavage products, with a 

 consequent reduction of total hydrophilic power. 



Fifth series: With leg miiscle. The conditions of this experi- 

 ment were practically the same as those for the fourth series of 

 tests. The volumes of alcohoP and water were 500 c.c. 



Data showing especially the amounts of non-coagulable nitrogen front edematous 



tissues and front corresponding control tissues which were 



prevented front becoming bloated 



® Preliminary treatment with hot alcohol in these experiments (III-V) 

 caused hardening and decided loss of weight. Immersion in water afterward, 

 for three days or more, failed with one exception to restore the original weight 

 of the tissue. 



* We determined the total nitrogen in each extract with intention to express 

 our "non-coagulable nitrogen" values in terms of their proportion of the total 

 nitrogen in the corresponding extracts. We abandoned this purpose after it 

 became evident that the figures for total nitrogen in the extracts did not provide 

 a reliable basis for the comparisons, although the data thus obtained warranted 

 the general conclusions we have drawn from the figures in this table. 



§ The " edema period " for each of the first three series was 24 hours. 



t The letters c and e indicate respectively the control and edematous por- 

 tions. In the table the data for the edematous portions are printed in heavy- 

 faced figures. 



II The " edema period " for the last two series was 72 hours. 



