544 



Ensymes as Factors in Edema 



[June 



TABLE 2 (contintied) 



Experiment p. Weight of fibrin in each mixture: 6 grams. Concentration 

 of sodium carbonate, 0.25^. Strength of the stock Solution of trypsin, 2^ (o.i 

 gram of product in 5 c.c. of water). 



Filtration of the liquid in the fourth mixture of the ninth experiment, 

 because of its rapid digestion, was begun at the 27th hour, when its volume was 

 143 C.C. 



Experiment 10. Weight of fibrin in each mixture: 6 grams. Concentration 

 of sodium carbonate, 0.50^. Strength of the stock Solution of trypsin, 1% (o.i 

 gram of product in 10 c.c. of water). 



Experiment 11. Weight of fibrin in each mixture: 3 grams. Concentration 

 of sodium carbonate, 0.25^. Strength of the stock Solution of trypsin, 2^ (0.1 

 gram of product in 5 c.c. of water). 



Filtration of the fourth fluid in this series was begun at the 30th hour, when 

 it was rapidly dissolving and its volume was 102 c.c. as recorded in the 32 hr. 

 column ; the first and third were filtered at the 32d hour ; the second was filtered 

 at the 45th hour, when its volume was 66 c.c. The fibrin in the fourth mixture 

 dissolved completely during the process of filtration. 







O.I 



0.2 

 0.3 



-3 



4 



133 



Experiment 12. Weight of fibrin in each mixture: 3 grams. Concentration 

 of sodium carbonate, 0.25^. Strength of the stock Solution of trypsin, 2^ (0.1 

 gram of product in 5 c.c. of water). 



The Solution of trypsin added to the third mixture was taken from a por- 

 tion of the stock Solution which had been thoroughly boiled and then cooled to 

 room temperature. 



o 



0.3 

 0.6 



10 



333 



tive hydrolysis which cannot be wholly avoided in experiments of 

 this kind with pepsin and trypsin. 



From the beginning of these studies it has been assumed that the 

 recorded volume of sediment in experiments of this kind cannot 



