GOLGI IN HELIX 117 



grounds of technique. Hirschler (vide 11), for example, found 

 that impregnation of the nuclear membrane could be obtained on 

 occasion. All such criticisms were done away with, however, in 

 the second paper by Nassonov (92), for they ^fere based on the 

 fact that the impregnated structure demonstrated by Nassonov 

 lay close or was definitely apposed to the wall of the contractile 

 vacuole, and therefore deposition of silver or osmium might occur 

 at the surface and produce an artefact. 



In Chilodon sp. the impregnated region is in the form of a ring 

 or girdle encircling the vacuole. There is no question of the im- 

 pregnation of a general vacuole-cytoplasm surface. Furthermore, 

 in DogieleUa sphcerii the apparatus (as it may now be confidently 

 termed) is not only in the form of a ring but stands well away from 

 the vacuole (Fig. 58, D, E). The ring appears flattish in section 

 and is composed of a lattice work of fairly fine texture. Nassonov 

 compares it with the condition found in the cells of the epidermis 

 of the gills of the axolotl larva, with which, from his illustrations, 

 it is practically identical. 



It may be taken for granted therefore that in those protozoa 

 possessed of a contractile vacuole the Golgi apparatus of the 

 metozoa is represented by a lipoidal substance in the neighbour- 

 hood of the walls of that vacuole. This homology, important as it 

 is from the morphological aspect, is doubly so from the physio- 

 logical or functional point of view. 



To turn now to other work described at about this time, 

 we find that a considerable amount of unconnected data and 

 observations were made. Brambell, in 1923 (14), describes the 

 behaviour of the Golgi apparatus in the neurones of Helix aspersa. 

 The apparatus consisted of rodlets in a homogeneous argentophil 

 cloud round the nucleus. In the large neurones this cloud was 

 almost absent on the side of the nucleus near the axon, and in 

 small neurones the cloud was confined to the side of the nucleus, 

 well awav from the axon base. The so-called Tigroid bodies 

 (Nissle granules) were always associated with the Golgi bodies. 

 It is, of course, well known that the Golgi apparatus usually under- 

 goes hypertrophy when repairs are being carried out to the nerve 

 fibres, but Bowen (11) drew attention to the general agreement of 



