158 ZOOLOGY 



similar to that of the parabasal bodies. King in Anoplophrya 

 hasili finds Golgi bodies consisting of distinct cavities in deeply 

 staining substance (71). Although they have no connection with 

 the cilia, King states that they are very like the bodies in Ojpalina. 

 The mitochondria are evenly distributed and quite distinct. The 

 homologies are, however, still considered doubtful. These 

 " Golgi bodies " can be seen in living preparations stained in 

 neutral red, and as we have shown previously, the vacuome is not 

 always closely connected with the true Golgi apparatus (although 

 it may be so in the Protozoa). 



We are on safer ground in considering the results of Nassonov in 

 ciliates and flagellates (91, 92). He has examined forms possessed 

 of contractile vacuoles, and has described the osmiophil and argeh- 

 tophil substance found apposed to the wall of these vacuoles (and 

 their contributory canals) as the Golgi apparatus (Fig. 58). 

 Certainly the appearance of these structures is similar to the 

 metazoan Golgi, particularly those in Chilodon and Dogiellela 

 (Fig. 58, D, E), where the apparatus is not too close to the vacuole, 

 but encircles it like a ring. Perhaps the most convincing evi- 

 dence is that of function, for, granted that these bodies are not 

 artefacts, then their relation to excretion is very similar to the 

 relation of the metazoan Golgi apparatus to secretion. (A 

 complete analysis of Nassonov' s work is given in the section on 

 the Function of the Golgi Apparatus.) 



Mention has already been made of Horning's work on the 

 mitochondria of Protozoa (59, etc.). Apart from the use of a 

 specific dye for the identification of these inclusions, the main 

 interest of his work undoubtedly lies in their relation to the food 

 vacuoles of Amoeba. Apparently the food particles are not sur- 

 rounded by a watery vacuole when they are engulfed. These 

 vacuoles appear only after mitochondria have adhered to the 

 surface of the food particles. His observations that the mito- 

 chondria appear to be unable to pierce the vacuoles when once 

 they have been formed cannot admit of any other plausible 

 explanation. Observations intra vitam with a dye have thus a 

 great advantage over other methods. 



The peculiar distribution of these bodies also appears to be in 



