186 BOTANY 



showed that one loop of the thread was definitely in contact with 

 this inclusion. Other nuclei, which present a similar stage of 

 development, show a loop of the thread attached to a darkly 

 staining oval body at the periphery of the nucleolus and no crystal 

 occlusions are simultaneously present. With the loosening of the 

 thread from synizesis, the peripheral darkly staining body becomes 

 considerably larger and more conspicuous and the connecting 

 threads still in contact with the nucleolus are very constantly 

 associated with it. Except for the presence of this deeply staining- 

 body (nucleolar body) the nucleolus is apparently homogeneous 

 at this stage. It would appear that this nucleolar body is derived 

 from the crystal occlusion of the resting nucleolus. With the 

 inception of the second contraction, the nucleolus frequently shows 

 budding and partial fragmentation, and the extruded portions 

 remain in the nuclear cavity as additional nucleoli and the 

 remaining portion of the nucleolus at the periphery becomes very 

 vacuolated in appearance. During the passage of the nucleolus 

 to the periphery of the contracted reticulum, several small 

 nucleolar can be seen. Their appearance suggests that the 

 large crystal body of the nucleus has undergone fragmentation. 



The fact that the time of association between the reticulum and 

 the nucleolar body coincides with the time of the chromatic thread 

 formation, suggests that the nucleolar body is of importance in the 

 passage of chromatic material to the linin thread. In the open 

 spireme stage, the nucleolar body is considerably larger than in the 

 early stages of synizesis. This proves that it cannot be the actual 

 substance of the original nucleolar body alone which is passed 

 on to the thread, but suggests rather that the function of the body 

 is that of an elaborating organ, and that it transfers elaborated 

 material to the thread with which it is in contact. 



Similarly, Gates and Latter discovered very much the same state 

 of affairs in Lathrcea. Unlike Lathyrus and most other forms, the 

 nucleolus never became crescent-shaped against the nuclear 

 membrane, but remained approximately spherical throughout the 

 prophase stages. Again a constant association was found of the 

 post-synizetic reticulum with one or more nucleolar bodies as in 

 Lathyrus, and crystal bodies were also found to be present. 



