IG STANDARDIZATION OF STAINS 



ments as to how the stains should be tested as to their behavior for biological 

 purposes and state the results to be expected from these tests. In every case 

 these specifications have been made to harmonize with the tests as actually per- 

 formed by the Stain Commission. 



2. The formulae given in the National Formulary, in "Biological Stains" and 

 in the "Manual of Methods for the Pure Culture of Bacteria," pubhshed by the 

 Society of American Bacteriologists, have been compared and critically studied 

 with the object of making them identical in all three. 



ABBREVIATIONS 



1 M (Greek letter for micron) = 1/lOOOth part of a millimeter (mm.) = 0.001 mm. = 10"' 



mm. = 10,000 A = approximately l/25,000th of an inch. 

 1 mn (millimicron) = 1/lOOOth part of a micron = 1/1, 000 ,000th part of a mm. = 10"^ mm. 



= 0.001 M = 10 A. 

 1 A (Angstrom unit) = 0.1 m.u = 0.0001 n = 10"^ mm. 

 1 nn (micromicron) = 1/1, 000 ,000th part of a micron = 1/1, 000,000,000th part of a mm. = 



10-» mm. = 0.000,001 m = 10"^ A. 

 1 Kg. = approximately 2.2 lbs. 



1 gm. = 10-3 Kg.^ 0.001 K., 1000 mgm., 1,000,000 /xg. 

 1 mgm. = 10-« Kg., 10-3 gm., 1000 fig. 

 1 pg. = l-y = 10-« Kg., 10-6 gru.^ 10-3 mgm. 



N NaCl is normal solution of sodium chloride, see Normal Solution. 

 M HCl is molecular solution of hydrochloric acid, see Molecular Solution. 

 M = mole. 

 mM = millimole. 

 ME = milligram equivalent. 



1 ml (milliliter) = l/l,000th part of a liter = 1 cc. (approx.). 

 CI 76 means that the number of a dye is 76 in the Colour Index of the Society of Dyers and 



Colourists. 

 CC. given after a dye signifies that it has been certified by the Biological Stain Commission! 



The following publications are simply referred to by author, or senior author, 

 or editor's name and page number (cf. Conn, p. 26). 



Bensley, R. R. and S. H., Handbook of Histological and Cytological Technique, Univ. 



Chicago Press, 1938, 167 pp. 

 Bourne, G., Cytology and Cellular Physiology, Oxford: Clarendon Press, 1942, 296 pp. 

 Conn, H. J., Biological Stains, Geneva, N. Y.: Biotech Publications, 1940, 308 pp. 

 CowDRY, E. v., Textbook of Histology, Philadelphia: Lea & Febiger, 1938, 600 pp. 

 Craig. C. F., Laboratory Diagnosis of Protozoan Diseases, Philadelphia: Lea & Febiger 



1942, 349 pp. 

 Downey, H., Handbook of Hematology, New York: Hoeber, 1938, 3136 pp. 

 Emig, W. H., Stain Technique, Lancaster: Science Press Printing Co., 1941, 75 pp. 

 Glasser, O. (Editor), Medical Physics, Chicago: Year Book Publishers, 1944, 1744 pp. 

 Lee, Bolles, The Microtomists' vade-mecum. Philadelphia: P. Blakiston's Son & Co. 



(Tenth Edition, Edited by J. B. Gatenby and T. S. Painter, 1937, 784 pp.) 

 Lison, L., Histochemie Animale, Paris: Gauthier-Villars, 1936, 320 pp. 

 Mallory, F. B., Pathological Technique, Philadelphia: Saunders, 1938, 434 pp. 

 McClung, C. a.. Microscopical Technique, New York: Hoeber, 1938, 698 pp. 

 Simmons, J. S. and Gentzkow, C. J., Laboratory Methods of the United States Army, 



Philadelphia: Lea & Febiger, 1944, 823 pp. 

 Stitt, E. R., Clough, p. W. and M. C, Practical Bacteriology, Haematology, and Animal 



Parasitology, Philadelphia: Blakiston, 1938, 961 pp. 



