ALVEOLAR EPITHELIUM 



23 



AMYLASE 



H., Anat. Rec, 1935, 64, 41-49). Use 

 guinea pigs. Silver Citrate sol. (which 

 see) is injected intx) lung substance by 

 hypodermic syringe, the roots of the 

 lung being first ligated, until the lung 

 is moderately distended. Cut out 

 pieces, fix in 10% formalin, imbed in 

 paraffin or celloidin, section, develop 

 with dilute photographic developer and 

 counterstain or examine unstained. 

 The margins of the cells are blackened. 

 For the most delicate results a slow 

 acting, fine grain developer such as the 

 following should be used : phenyl hy- 

 drazine hydrochloride, 1 gm., sodium 

 sulphite (anhydrous), 10 gm.; aq. dest., 

 100 cc. Caution: Phenyl hydrazine 

 hydrochloride is extremely toxic to 

 some people producing skin reactions. 

 (Revised by R. D. and S. H. Bensley, 

 April 18, 1946.) 



Alveolar Pores of the lung (Macklin, C. C. 

 Arch. Path., 1936, 21, 202-216). For- 

 malin (10%) and Zenker-formalin are 

 among the fixatives suggested. The 

 fixative is injected into the trachea or 

 bronchus at a gravity pressure of 4-6 

 inches until the lungs are moderately 

 distended. During this operation they 

 are covered with physiological salt solu- 

 tion. The lungs are then immersed in 

 fixative for days or even weeks. Slices 

 about 1 cm. thick are cut, imbedded in 

 soft paraffin and sections are made at 

 100;u or more. Resorcin-fuchsin and 

 other stains may be used. The blood 

 in the capillaries is a useful guide. The 

 pores can be identified by their rounded 

 edges (Revised by C. C. Macklin, 

 Dept. of Histology, University of 

 Western Ontario, London, Canada, 

 1946). 



Alzheimer's Modification of Mann's eosin- 

 methyl blue for neuroglia and degenerate 

 nerve fibers as given by IVIallory (p. 245) 

 is abbreviated. Fix thin slices, 14 days, 

 in Weigert's Neuroglia Mordant + 10% 

 of formalin. Wash 8-12 hrs. in running 

 water. Mordant lOyu frozen sections 

 2-12 hrs. in sat. aq. phosphomolybdic 

 acid. Wash 2 changes aq. dest. Stain 

 in Mann's Eosin Methyl Blue 1-5 hrs. 

 Wash quickly in aq. dest. until color 

 "clouds" are no longer given off. 

 Treat with 95% alcohol until gray matter 

 becomes light blue and white matter 

 pink or bright red. Dehydrate quickly 

 in absolute alcohol, clear in xylol and 

 mount in balsam. Normal axis cylin- 

 ders, purple or deep blue; degenerating 

 ones, red; neuroglia fibers, dark blue; 

 and neuroglia cytoplasm, pale blue. 

 Mallory states that change from blue to 

 red staining of axis cylinders occurs as 

 soon as 48 hrs. after experimental lesion. 



Amanil Garnet H., see Erie Garnet B. 



Amaranth (CI, 184) — azo rubin, Bordeaux, 

 Bordeaux SF, fast red, naphthol red S, 

 C or O, Victoria rubin 0, wool red — An 

 acid mono-azo dye used long ago by 

 Griesbach, H., Zeit. wis. mikr., 1886, 

 3, 358-385 to color axis cylinders. 



Amebae, see Endamoeba. 



Amethyst Violet (CI, 847)— heliotrope B, 

 iris violet — It is a basic azin dye of little 

 importance to histologists. 



Amino Acids, see Alloxan Reaction, also 

 Schmidt, C. L. A., The Chemistry of 

 the Amino Acids and Proteins. Spring- 

 field, Charles C. Thomas, 1938, 1031 pp. 



Aminoacridines, some are strong antiseptics, 

 do not stain skin (Albert, A. and 

 Ritchie, B., J. Soc. Chem. Ind., 1941, 

 60, 120). 



Amitosis is direct nuclear division by con- 

 striction without formation of a chro- 

 matin thread. No special technique 

 required. Study of embryonic mem- 

 branes and of bladder of mouse (Dogiel, 

 A. S., Arch. f. Mikr. Anat., 1890, 35, 

 389-406) is suggested. 



Ammonia Carmine (Ranvier). A suspen- 

 sion of carmine in water, with slight 

 excess ammonia, is allowed to evaporate 

 in air. If it putrefies so much the 

 better. Dissolve the dry deposit in 

 aq. dest. and filter (Lee, p. 145). 



Ammonium molybdate, as mordant for 

 Mann's stain and Weigert-Pal (Perdrau, 

 J. R., J. Path. & Bact., 1939, 48, 609- 

 610). 



Amphinucleolus (G. amphi on both sides). 

 A nucleolus which is double consisting of 

 both acidophilic and basophilic parts, 

 the former is usually a central core and 

 the latter plastered on its surface. 



Amphophilic, see Staining. 



Amyl Acetate, as solvent for imbedding 

 tissues (Barron, D. H., Anat. Rec, 



1934, 59, No. 1 and Suppl., 1-3); as a 

 clearing agent for embryological material 

 (Drury, H. F., Stain Techn., 1941, 

 16, 21-22). 



Amyl Alcohol. Merck lists 3, commercial, 

 normal and tertiary. It mixes with 

 95% alcohol and with xylol. Hollande 

 (A. C, C. rend Soc. de Biol., 1918, 81, 

 223-225) was the first to recommend 

 amyl alcohol as a substitute for absolute 

 alcohol in the dehydration of specimens 

 stained by the Romanovsky and Giemsa 

 techniques. 



Amyl Nitrite. McClung (p. 620) says that 

 this may serve as a dilator of peripheral 

 capillaries when a complete injection 

 of small blood vessels is required. Add 

 it to the ether at time of anesthetization. 



Amylase, micromethod for (Pickford, G. E. 

 and Dorris, F., Science, 1934, 80, 317- 

 319). This was later used with marked 

 success by Dorris (F., J. Exp. Zool., 



1935, 70, 491-527) in a study of relation 



