BARIUM 



38 



BENDA'S 



by hypodermic injection of formalin 

 and subsequent immersion in it. Take 

 x-ray photographs of the radiopaque 

 barium. 



Basal Bodies of cilia (Wallace, H. M., 

 Science, 1931, 74, 369-370). Fix in 

 Zenker (containing acetic) or in Zenker- 

 formalin (90 cc. Zenkers + 10 cc. 10% 

 formalin). Mount paraffin sections 5/x 

 thick. After very light staining with 

 hematoxylin and thorough washing in 

 tap water dip in 0.5% aq. eosin 

 (Grubler's ivasserlich. If not available, 

 use Eosin Y.) ^ min. and wash quickly 

 in large volumes of water. Make up 

 stain by adding 9 parts sat. aq. methyl 

 violet (Grubler's 6B only. If not 

 available, use CC. which is 2B.) to 1 

 part abs. alcohol 33 cc. ; aniline oil 9 cc. 

 + methyl violet in excess. Stain is 

 best 3-8 days after mixing but the two 

 solutions can be kept separately. After 

 staining sections for 2 hrs. wash well in 

 tap water, treat with Lugol's iodine 

 10-15 min. and repeat the washing. 

 Blot with filter paper. Differentiate in 1 

 part aniline oil + 2 parts xylol. Wash 

 in several changes of xylol and mount in 

 balsam. Basal bodies deep purple, 

 nuclei dark blue. Good also for intra- 

 cellular bacteria and fibrin. 



Basic Brown, G, GX, or GXP, see Bismark 

 Brown Y. 



Basic Dyes, see Staining. 



Basic Fuchsin — anilin red, basic rubin, and 

 magenta (CI 676 or 677)— Commission 

 Certified. The tri-amino tri-phenyl 

 methane dyes bearing this name are 

 mixtures of pararosanilin, rosanilin and 

 magenta II in varying proportions. 

 They are employed for a great many 

 purposes. Basic fuchsin in a cytologi- 

 cal technique for anterior pituitary is 

 described by Faire, W. R., and Wolfe, 

 J. M., Anat. Rec, 1944, 90, 311-314. 

 New fuchsin (CI 678) is a different com- 

 pound. It is the deepest in color of 4 

 dyes and pararosanilin is the lightest. 



Basic Lead Acetate used as fijiative for 

 Tissue Basophiles. 



Basic Rubin, see Basic Fuchsin. 



Basophila Erythroblasts, see Erythrocytes, 

 developmental series. 



Basophile Leucocyte (mast-leucocyte, blood 

 mast cell). Least numerous granular 

 leucocyte ; percentage about 0-1 ; 

 slightly smaller (8-10m) than other 

 types; nucleus spherical or slightly 

 lobated, faintly staining and centrally 

 placed; specific granules only slightly 

 refractile, basophilic, large, variable and 

 less numerous than in other types; 

 function unknown. This cell is difficult 

 to study in fresh preparations of pe- 

 ripheral blood because it is so scarce. 

 Smears colored by the usual methods 



(Giemsa, Wright, etc.) are satisfactory. 

 The basophilic granules appear to be 

 particularly soluble in water. Doan 

 and Reinhart (C. A. and H. L., Am. J. 

 Clin. Path., 1941, 11, Tech. Suppl. 5, 

 1-39, with beautiful colored plates) 

 reconamend supravital staining with 

 neutral red and janus green. There is 

 difference of opinion as to whether the 

 oxidase and peroxidase reactions are 

 positive (Michels, N. A. in Downey's 

 Hematology, 1938, 1, 235-372). See 

 Tissue Basophiles. 



Basophilic, see Staining. 



Bell's Method for fixing and staining of fats 

 as described by the Bensleys (p. 114). 

 Intracellular fats are mobilized by heat 

 to form droplets which are chromated 

 and later stained. Consequently the 

 preparations show these fats, in addition 

 to other microscopically visible fat, but 

 not their true distribution in the cells. 

 Fix for 10 days at 45-50 °C. in 10% aq. 

 potassium bichromate 100 cc. -f 5 cc. 

 acetic acid. Imbed and make paraffin 

 sections as usual. Pass them down to 

 absolute alcohol. Stain with freshly 

 prepared Sudan III 10 min. Rinse off 

 in 50% alcohol and pass to water to arrest 

 action of alcohol. Counter-stain with 

 Delafield's Hematoxylin. Wash in 

 water, differentiate in acid alcohol, wash 

 in water again and mount in Glycerine 

 Jelly. 



Benda's Method of crystal violet and 

 alizarin for mitochondria. Fix in Flem- 

 ming's fluid 8 days (see Flemming's 

 Fluid). Wash in water 1 hr. Then 

 half pyroligneous acid and 1% chromic 

 acid, 24 hrs. 2% potassium bichromate, 

 24 hrs. Wash in running water 24 hrs. 

 Dehj^drate, clear, imbed in paraffin and 

 cut sections at 4^t. Pass down to water 

 and mordant in 4% iron alum 24 hrs. 

 Stain amber-colored sol. sodium sulpha- 

 lizarinate made by adding sat. ale. sol. 

 to water, 24 hrs. Blot with filter paper 

 and color in equal parts crystal violet 

 sol. and aq. dest. (The sol. consists of 

 sat. crystal violet in 70% ale. 1 part, 

 ale. 1 part and anilin water 2 parts.) 

 Warm until vapor arises and allow to 

 cool 5 min. Blot and immerse in 30% 

 acetic acid 1 min. Blot, plunge in abs. 

 ale. until but little more stain is ex- 

 tracted, clear in xylol and mount in 

 balsam. The mitochondria are stained 

 deep violet in a rose background. The 

 colors are more lasting than in Altmann 

 preparations. This is one of the classi- 

 cal techniques of histology but it is 

 difficult. For colored illustrations see 

 Duesberg, J., Arch. f. Zellforsch., 1910, 

 4, 602-671. 



Benda's stain for fat necrosis. See Fisch- 

 ler's modification. 



