CARBOL-ANILIN FUCHSIN 



52 



CAREY'S 



Carbol-Anilin Fuchsin methylene blue 

 method for Negri bodies (Goodpasture, 

 E. W., Am. J. Path., 1925, 1, 547-582). 

 Fix in Zenker's fluid 24 hrs. (not Helly's 

 fluid). Color for 10-30 min. in mixture 

 made by adding 1 cc. of pure phenol and 

 1 cc. of anilin oil to 100 cc. of stock 0.5% 

 basic fuchsin in 20% alcohol. Wash in 

 running water, blot with filter paper and 

 decolorize with 95% alcohol until sec- 

 tions become pink. Then wash in water 

 and stain with Loeffler's methylene 

 blue, 15-60 sec. Wash again in water. 

 Dehydrate and destain for few sec. in 

 absolute alcohol, clear in xylol and 

 mount in balsam. Negri bodies, crim- 

 son; background, blue. Also excellent 

 for Borrel Bodies. 



Carbol-Crystal Violet. Because the solu- 

 tions as prescribed in Nicolle's original 

 formula for carbol gentian violet tend 

 to gelatinize, the following formula is 

 recommended by Conn, H. J., Stain 

 Techn., 1946, 21, 31-32: Mix solution of 

 0.4 gm. crystal violet C. C. in 10 cc. 

 95% ethyl alcohol with solution of 1 gm. 

 phenol in 100 cc. aq. dest. 



Carbol-Fuchsin. The original formula of 

 Ziehl has been much modified. Ziehl- 

 Neelsen is sat. ale. basic fuchsin, 10 cc. ; 

 5% aq. carbolic acid, 90 cc. Verhoeff 

 (F. H., J.A.M.A., 1912, 58, 1355) advises 

 basic fuchsin, 2 gm.; abs. ale, 50 cc. ; 

 melted carbolic acid crj^stals, 25 cc. 

 McClung (p. 136) suggests mixing 10 cc. 

 3% basic fuchsin (90% dye content) 

 with 95 cc. 5% aq. phenol. The im- 

 portant thing is the character of the 

 fuchsin not its concentration relative to 

 carbolic acid. Carbol-fuchsin is em- 

 ployed in stains for Acid Fast Bacilli. 

 Deipolli, G. and Pomerri, G., Mon. 

 Zool. Ital., 1938, 49, 123-124 have ad- 

 vised its use as follows for Niss! Bodies. 

 Fix small pieces in 95-98% alcohol or in 

 10% formalin water or in physiological 

 saline 24 hrs. or longer. Stain deparaf- 

 finized sections 3-4 min. in carbol- 

 fuchsin (basic fuchsin, 0.2 gm.; cone, 

 phenol, 1 cc; 95% ale, 2 cc; aq. dest. 

 20 cc) 2.5 cc; aq. dest., 100 cc; glacial 

 acetic acid, 0.5 cc Wash rapidly in aq. 

 dest. and destain in: aq. dest., 100 cc; 

 formalin, 1 cc. ; glacial acetic acid, 1 cc. 

 Wash in aq. dest., dehydrate in alcohols, 

 clear in xylol and mount in neutral 

 balsam. Nissl bodies and nucleoli dark 

 red, rest unstained. 



Carbol-Thionin, see King's. 



Carbol-Xylol. Xylol saturated with car- 

 bolic acid crystals. After using it for 

 clearing celloidin sections, wash quickly 

 in xylol before mounting them in 

 balsam. 



Carbon from inspired air occurs abundantly 

 in lungs and bronchial lymph nodes. 

 It may be transported to the great blood 



filters (spleen and liver) where it is 

 distinguishable by its black color and 

 by its insolubility in cone sulphuric 

 acid which dissolves all other body 

 pigments. Fine suspensions of carbon 

 are of great service as vital stains to 

 demonstrate phagocytosis. See Hig- 

 gins' Ink and Lampblack. 



Carbonic Anhydrase. This can be localized 

 in the oxyntic (or parietal) cells of the 

 fundus of the stomach. Davenport, 

 H. W., Am. J. Physiol., 1940, 128, 

 725-728; 129, 505-514 employed an 

 adaptation of Linderstr0m -Lang's tech- 

 nique and observed that in rats and cats 

 the parietal cells contain 5 to 6 times as 

 much of the enzyme as red blood cells 

 while the peptic cells are free from it. 

 A microspectroscopic method for demon- 

 stration of carbonic anhydrase within 

 erythrocytes depends on the action of 

 methemoglobin as an indicator which 

 changes both its color and pattern of 

 absorption spectrum with change of pH 

 from 6.5-9.5 (Keilin, D. and Mann, T., 

 Nature, 1941, 148, 493-i96). For data 

 on the distribution of this enzyme in 

 lower forms, see Blaschko and Jacobson 

 (Bourne, p. 200). 



Carey's method for motor end plates is an 

 adaptation for his study of their ameboid 

 motion (Carey, E. J., Anat. Rec, 1941, 

 81, 393-413) of Wilkinson's (H. J., Med. 

 J. Austral., 1929, 2, 768-793). Modifica- 

 tion of Ranvier's gold chloride technique. 

 — Written by E. J. Carey, Dept. of 

 Anatomy, Marquette University School 

 of Medicine, Milwaukee, Wis. 



1. Remove any muscle from rat or 

 chameleon from its origin to insertion 

 while the animal is under ether or nemo- 

 butal anesthesia. Using a very sharp 

 knife cut the muscle quickly into pri- 

 mary pieces, 0.5 cm. long, and 0.5 cm. 

 thick, following the long axis of the 

 muscle fibers. Then cut the primary 

 pieces longitudinally into thin strips 

 1 to 2 mm. wide. 



2. Soak strips in freshly prepared 

 filtered lemon juice for 5 to 10 min. 

 until they become clear or translucent. 

 Rinse in cold tap water 4 to 5 times. 



3. Place strips in 1% aq. gold chloride 

 at 30°C. using at least 10 times the 

 volume of gold chloride solution to each 

 volume of muscle. While muscle is in 

 gold chloride solution, stir at least once 

 a min. The time for the optimum im- 

 pregnation of gold varies in the different 

 muscles of the same animal at a rela- 

 tively constant rate, for example, the 

 sternocleido-mastoid muscle of the 

 normal rat requires 16 min.; the pec- 

 toralis major, adductors of the thigh, 

 and biceps femoris, 13 min.; and the 

 gastrocnemius, tibialis anterior, and 

 the intercostal muscles 10 min. After 



