CORNEA 



74 



GROSSMAN'S 



and cytoplasm well shown and espe- 

 cially Descemets membrane. 



Cornyebacterium Diphtheriae. Evaluation 

 of methods for staining metachromatic 

 granules (Morton, H. E., Stain Techn., 

 1942, 17, 27-29). See Gobar, M. A., J. 

 Bact., 1944, 47, 575, also Diphtheria 

 Bacilli. 



Coronary Arteries. Their distribution may 

 be demonstrated by injection of the 

 easily recognizable fluids listed under 

 Blood Vessels. Owing however to their 

 great importance it is well to mention 

 two special adaptations of the said fluids. 

 Gross (L., The Blood Supply of the 

 Heart in its Anatomical and Clinical 

 Aspects. New York: Hoeber, 1921) 

 employed injections of barium sulphate 

 suspensions in gelatin followed by x-ray 

 photographs; while Spalteholz (W., 

 Die Arterien der Herzwand, etc., 

 Leipsig: Herzel, 1924) used injections 

 of cinnabar and other pigments likewise 

 in gelatin which were later cleared by 

 his method. Ehrich, Chapelle and Cohn 

 (W., C, and A. E., Am. J. Anat., 1931, 

 49, 241-282) found the latter technique 

 preferable. Celloidin injections also 

 give good results. Histological demon- 

 stration of the blood supply of the 

 coronaries is described under Vasa 

 Vasorum. 



Corpora Amylacea, see Prostate. 



Corrosion Preparations. In them the struc- 

 tures to be demonstrated are left while 

 all the surrounding tissue is corroded 

 and washed away, for instance Celloidin 

 and Neoprene injections. 



Corrosive Sublimate, see Mercuric Chlo- 

 ride. 



Corti, organ of, see Ear. 



Cortin (interrenalin), hormone of adrenal 

 cortex. 



Cotton Blue, see Anilin Blue, Methyl 

 Blue. Sec Fungi. 



Cotton Corinth G, see Erie Garnet B. 



Cotton Red, see Safranin O. 



Cotton Red, A, B, or C, see Congo Red. 



Cotton Red 4B, see Benzopurpurin 4B. 



Cover Glasses, see Cleaning. 



Cresol Red. See Hydrogen Ion Indicators. 



Creosote (Beechwood) is an important 

 clearing agent for celloidin sections. 

 It is a mixture of phenols, mainly 

 guaiacol and creosol. 



Cresyl Blue 2RN, or BBS, see Brilliant 

 Cresyl Blue. 



Cresyl Violet — cresylecht violet (cresyl fast 

 violet) — Commission Certified. A basic 

 oxazin dye. A technique for its use 

 (or that of toluidin blue) in studies on 

 the cytoarchitectonics of the nervous 

 system is proposed by Landau, E., 

 Bull. d'Hist. AppL, 1934, 11, 44^6. 

 As a stain for nerve cells in celloidin 

 sections (Tress, G., and M., Stain Tech., 

 1935, 10, 105-106). Wash low viscosity 



nitrocellulose (celloidin) sections of 10% 

 formalin fixed tissues in aq. dest. Stain 

 for 30 min. at 50°C. in cresyl violet, 

 0.5 gm.; aq. dest., 100 cc. ; glacial acetic 

 acid, 4 drops (filtered before using). 

 Wash in aq. dest. Differentiate in 70% 

 alcohol until most of stain leaves cel- 

 loidin. Completely immerse for 2-5 

 min. in: chloroform, 60 cc; abs. ale, 

 10 cc; and ether, 10 cc. Almost no 

 destaining of cells occurs but stain is 

 removed from background. Differen- 

 tiate in 100 cc. 95% ale. -f 4 drops 1% 

 aq. hydrochloric acid but stop while 

 cells are a little darker than desired. 

 Neutralize sections in 90% alcohol -f- 

 a little sodium bicarbonate. Wash in 

 95% alcohol to remove the bicarbonate. 

 Complete dehydration in 2 changes n 

 butyl alcohol. Clear in 4 changes xylol 

 and mount. See Kallichrom. Note: 

 There are two different dyes sold as 

 cresyl violet: (1) The CC. product 

 (Nat. Anilin, Mfgrs.; see Conn, 1940, 

 p. 93) which is good in biopsy work; 

 (2) The Grubler product (also sold by 

 Coleman and Bell, but not on the market 

 during the war) which is needed in 

 neurological work, cf. Tress, above. 



Cresylecht Violet, intensification of meta- 

 chromatic properties (Williams, B. G. 

 R., J. Lab. & Clin. Med., 1934-35, 20, 

 1185-1187). 



Crime Detection Techniques. These are of 

 course legion. Many of them are mi- 

 croscopic and involve identification of 

 materials. See for example, Hair, 

 Semen Stains and Hemoglobin. In re- 

 spect to the latter the object is to deter- 

 mine whether blood is human by pre- 

 cipitin tests and to which group it be- 

 longs by detection of agglutinins as is 

 well described by Schiff, F. and Boyd, 

 W. C, Blood Grouping Technic New 

 York: Interscience Publishers, Inc., 

 1942, 248 pp. The identification of 

 metals, such as chips from a razor blade, 

 by spectroscopic examination is often 

 conclusive, see Histospectrography. 

 Cracks in metal surfaces can be de- 

 tected with astonishing delicacy b}'' the 

 Magnaflux. An interesting elementary 

 account of Crime Detection Techniques 

 is provided bv Hoover, J. E., Scientific 

 Monthly, 1945, 60, 18-24. 



Croceine Scarlet, see Biebrich Scarlet, 

 water soluble. 



Grossman's modification of Mallory's con- 

 nective tissue stain (Grossman, G., 

 Anat. Rec, 1937, 69, 33-38). Deparaf- 

 finize sections of Zenker fixed material. 

 Lugol's iodine, 5 min. Rinse in 70% 

 alcohol, several changes. Wash 10 min. 

 in running water. Overstain nuclei in 

 Mayer's acid Hemalum or Weigert's 

 Iron Hematoxylin. Wash in running 

 water 10 min. Stain for 1 min. or more 



