EYES 



93 



FECES 



also easier in celloidin because one can 

 see through it fairly well. 



If, on the other hand, preparations are 

 needed of small parts of the eye these 

 parts should be carefully dissected out 

 and the paraffin technique employed. 

 Much time will be saved by following 

 the excellent suggestions made by S. L. 

 Polyak, The Retina. Univ. of Chicago 

 Press, 1941, 607 pp. and by G. L. Walls 

 (Stain Techn., 1938, 13, 69-76). 



Dr. Polyak in a letter dated April 19, 

 1946 calls attention to the advisability 

 of soaking celloidin blocks in oil as first 

 described by Apdthy, S., Zcit. f. wis. 

 Mikr., 1912, 29, 464. The same method 

 is well presented by Kranse, R., Enzyk. 

 d. Mikr. Technik., 3rd edit., 1926, 1, 

 281. For the investigation of perme- 

 ability, oxidation-reduction potential, 

 enzyme sj^stems, and such properties, 

 see Friedenwald, J. S. and Stiehler, 

 R. D., Arch. Ophth., 1938, 20, 761-786. 

 Useful data are to be found in Kurzes 

 Handbuch der Ophthalmologie (Schieck 

 and Briickner, Berlin: Julius Springer, 

 1930, 1, 882 pp.) The Anterior Chamber 

 is a favourite site for tissue trans- 

 plantation. 



Frozen sections of bird's eyes. (Oak- 

 ley, C. L., J. Path. & Bact., 1937, 44, 

 365-368). Fix in 10% formol saline 4 

 days, in Miiller's fluid, 6 weeks in incu- 

 bator, or, in case speed is necessary, in 

 Perdrau's fluid 4 days. Incise large 

 eyes to aid penetration. Wash in run- 

 ning water at least 24 hrs. because 

 formalin and bichromate should be com- 

 pletely removed. Cut eye in half being 

 careful not to disturb various structures. 

 12.5% gelatin + 1% phenol over night, 

 25% 24 hrs. at 37°C. Employ at least 

 25 cc. for each half eye. Mount with 

 cut surface down in dish containing 25% 

 melted gelatin. Set overnight in run- 

 ning water or in icebox (not refrigera- 

 tor). Cut out block, trim away excess 

 gelatin. Harden in large amount 10% 

 formalin, 2-3 days, store in 4% formalin. 

 Before freezing soak 15 min. in tap 

 water. Freeze slowly, over-freeze and 

 then stain usual methods but carefully 

 avoid strong alcohols. They will stand 

 70% and 1% HCl provided washing in 

 water liaa been thorough. Use glycerin 

 jelly for mounting. 



Fahrenheit Temperature to Centigrade. 



Use the following relation: 



a (F°-32) =c° 



302 °F ± i (302 - 32) = a (270) = 150* C. 



.S-F ± S (5 - 32) = a (- 27) = - 15°C. 



- 13°F ± 5 (- 13 - 32) = i (- 45) = - 25''C. 



Fallopian Tubes (oviducts, uterine tubes). 

 References to many techniques will be 



found in C. G. Hartman's chapter in 

 Allen, Danforth and Doisy's Sex and 

 Internal Secretions. Baltimore : Wil- 

 liams and Wilkins, 1939, 1346 pp. 



Farrant's Medium. Gum arable, 30 gm.; 

 glycerin, 30 cc. ; arsenous oxide (arsenic 

 trioxide), 0.1 gm.; aq. dest., 30 cc. 

 (McClung, p.617). 



Fast Acid Blue R (CI, 760). An acid xan- 

 thene dye. Conn (p. 143) says that it 

 is almost the same as violamine 3B which 

 contains small amount of a red dye. See 

 Romell, L. G., Stain Techn., 1934, 9, 

 141-145 under Soil, bacteria. 



Fast Acid Green N, see Light Green SF 

 yellowish. 



Fast Blue B, OB, R, etc., see Indulin, 

 water soluble. 



Fast Blue 3R, see Naphthol Blue R. 



Fast Fuchsin G, see Chromotrope 2R. 



Fast Green FCF. Commission Certified. 

 Closely related to Light Green SF 

 yellowish and recommended as a sub- 

 stitute because it fades less. 



Fast Oil Orange II, see Oil Red O. 



Fast Red, see Amaranth. 



Fast Red B, BN or P, see Bordeaux Red. 



Fast Violet, see Gallocyanin. 



Fast Yellow (CI, 16)— acid yellow, fast 

 yellow FY, G, S, BG, etc.— An acid 

 mono-azo dye. Employed by several 

 investigators, see use by Wallart, J. and 

 Houette, C, Bull. d'Hist. Appl., 1934, 

 11, 404-407 in rapid tri chrome hematox- 

 ylin, acid fuchsin fast yellow method. 

 They used "Jaune solide G or GG 

 (Ciba). 



Fasting. Structural changes in human di- 

 gestive tract (Cowdry's Histology, 

 p. 305). 



Fat Blue B, see Victoria Blue B. 



Fat Blue 4R, see Victoria Blue 4R. 



Fat Ponceau, see Oil Red O. 



Fat Ponceau, see Sudan IV. 



Fat Ponceau G, see Sudan III. 



Fat Ponceau R or LB, see Sudan IV. 



Fats, see Lipids. 



Fatty Acids, see Lipids, examination with 

 polarized light, also lack of specificity 

 of blue color with Nile Blue Sulphate. 

 A review of the method of tagging fatty 

 acids with radioactive isotopes is given 

 by Bloor (W. R., Physiol. Rev., 1939, 

 19, 557-577). 



Feathers, see Ceresin imbedding. 



Feces. 1. To demonstrate ova of parasites 

 (Mallory, p. 301). If they cannot be 

 seen when a small bit of feces is mixed 

 with water on a slide attempt to concen- 

 trate them. To a small amount of feces 

 add sufficient sugar solution (common 

 granulated sugar, 500 gm., water, 360 cc, 

 phenol, 1%) to almost fill tube. Cover 

 and gently mix contents. Centrifuge 

 at 1000 r.p.m. 5-6 min. Remove ma- 

 terial from surface in wire loop and 



