HEMOPHILUS PERTUSSIS 



119 



HISTOSPECTROGRAPHY 



phosphomolybodic acid. Growth on a 

 special medium is advised (Lawson, G. 

 McL., J. Lab. & Clin. Med., 1939-40, 

 25, 435-438). 



Hemosiderin, soluble in acids and other 

 reagents used in histological technique. 

 After formalin fixation the order of 

 decreasing removal is oxalic, sulphuric, 

 nitric, formic and hydrochloric. Speed 

 of solution is but little influenced by age 

 of pigment (Lillie, R. D., Am. J. Path., 

 1939, 15, 225-239). See Iron, Di- 

 nitrosoresorcinol method. 



To demonstrate hemosiderin micro- 

 scopically pour on deparaffinized sec- 

 tions of freshly fixed tissue 1 part of 

 fresh 2% aq. potassium ferrocyanide 

 and 3 parts 1% aq. hydrochloric acid 

 heated to 60°-80°C. Thoroughly wash 

 in several changes of water. Counter- 

 stain in 0.1-0.5% basic fuchsin in 50% 

 alcohol, 5-20 min. Wash in water. 

 Pass through 95% and abs. alcohol and 

 xylol and mount in balsam. Nuclei 

 and hemofuscin, red; hemosiderin, blue 

 (J. E. Ash in Simmons and Gentzkow, 

 p. 744). See Iron and Hemofuscin, 



Heparin. A method for the histological 

 demonstration of heparin has been de- 

 scribed by Jorpes, E., Holmgren, H. and 

 Wilander, O., Ztsch. f. mikr. anat. 

 Forsch., 1937, 42, 279-301. It is based 

 on evidence that Tissue Basophiles 

 contain this substance. See also Anti- 

 coagulants. 



Heptaldehyde. An agent said by Strong, 

 L. C, Am. J. Cancer, 1939, 35, 401^07, 

 to produce liquefaction of spontaneous 

 mammary tumors of mice. It was not 

 helpful when injected into rat lepro- 

 mata (Cowdry, E. V. and Ruangsiri, C, 

 Arch. Path., 1941, 32, 632-640). 



Hermann's Fluid. 2% osmic acid, 4 cc; 

 1% platinum chloride, 15 cc. ; glacial 

 acetic acid, 1 cc. This resembles 

 Flemming's fluid and is a good cyto- 

 logical fixative. 



Herring Bodies, see Gushing, H., Proc. Soc. 

 Exp. Biol. & Med., 1932-33, 30, 1424- 

 1425. 



Hertzberg's Victoria Blue stain for elemen- 

 tary bodies is described by Seiffert, G., 

 Virus Diseases in Man, Animal and 

 Plant. New York: Philosophical Li- 

 rary, Inc., 1944, 332 pp. It is rather 

 like Gutstein's technique (see Ele- 

 mentary Bodies) except that Hertzberg 

 does not make up the stain with po- 

 tassium hydrate and Gutstein does not 

 destain in 1% citric acid. 



Herxheimer's solution for staining fat : 

 scarlet red (scharlach R, sudan IV), 

 1 gm. ; 70% alcohol, 50 cc. ; acetone C.P., 

 50 cc. See Sudan IV. 



Heterophile, see Staining. 



Hexuronic Acid as antiscorbutic factor 



(Harris, L. J., and Ray, S. N., Biochem: 

 J.1933, 27, 58-589). 



Hickson Purple, a disazo dye, giving in aq. 

 sol. a purple color to leucocytes and a 

 red color to erythrocytes introduced by 

 H. G. Cannan (J. Roy. Micr. Soc, 1941, 

 61,88-94). 



Higgins' Ink. This was apparently first 

 used as a vital stain by George Wislocki, 

 see Foot (McClung, p. 114). Dilute 

 with equal volume sterile aq. dest. 

 Warm and inject into marginal vein of 

 rabbit's ear 5 cc. daily for 3-4 days, then 

 every 3 days as long as desired. Since 

 the carbon is relatively insoluble it is a 

 simple matter to fix, imbed, section and 

 counterstain. Smaller amounts are to 

 be used for smaller animals, see Vital 

 Stains. 



Hirudinea, see Parasites. 



Hischler's Fluid, see Golgi Apparatus. 



Hiss's Method for capsule staining, see 

 Capsule. 



Histiocyte, a term without value as it 

 simply indicates a "tissue cell," often 

 applied to phagocytic cells of connec- 

 tive tissue. 



Historadiography is the x-ray photography 

 of tissues. By a special technique 

 Larmaque, P., Bull. d'Hist. AppL, 

 1937, 14, 1-16) rays emitted at a tension 

 of 50-100 KV having a length of 0, 12-0,2 

 A° are directed upon a section closely 

 applied to a particularly finely grained 

 emulsion. The absorption of the rays 

 by the section depends upon the density 

 of its parts. Total opacity of the tissue 

 to the rays is marked on the photo- 

 graphic negative by white, permeability 

 by black, and there are usually all grades 

 between the two. Subsequent magnifi- 

 cation of about 500 times is possible, but 

 is not advisable. Sections, not more 

 than 4 microns thick, of formalin fixed 

 tissues, are recommended. An illus- 

 trated description of the appearance of 

 epidermis, cartilage, artery wall, thyroid 

 and other tissues is provided by Tur- 

 chini (J. Bull. d'Hist. AppL, 1937, 14, 

 17-28). Historadiography may have 

 many uses in the measurement of 

 densities in different physiological states 

 and in study of the distribution of sub- 

 stances opaque to x-rays experimentally 

 introduced. In some cases great den- 

 sity may accompany high Viscosity. 



Histospectrography. This is a very valu- 

 able survey method for minerals in 

 tissues. See Policard, A., Protoplasma, 

 1933, 19, 602-629; Scott, G. H. and 

 Williams, P. S., Anat. Rec, 1935, 64, 

 107-127; Cowdry, E. V., Heimburger, 

 L. F., and Williams, P. S., Am. J. Path., 

 1936, 12, 13-29. Optic lens and cata- 

 racts have been analysed particularly 

 for iron, copper and zinc (Busnel, R. G., 



