INDULIN 



125 



IODINE 



Lynch, J. E., Zoit. f. wis. mikr., 1930, 

 46, 465-469; Cumley, R. W., Stain 

 Techn., 1935, 10, 53-56. 



Indulin Black, see Nigrosin, water soluble. 



Infra Red photography shows split appear- 

 ance of chromosomes (Ganesan, D., J. 

 Roy. Micr. Soc, 1939, 59, 75-78) and 

 gives better definition of epiphyseal 

 layers of normal and rachitic bone 

 (Siegel, L., Allen, R. M., McGuire, G. 

 and Falk, K. G., Am. J. Path., 1939, 15, 

 273-277). Guardabassi, M., C. rend. 

 Soc. de Biol., 1935, 118, 559-561 has 

 used this technique for alcohol fixed 

 sections of brain of rabid dog sensitized 

 with rubrocj^anine to demonstrate struc- 

 ture of Negri bodies. Transmission of 

 infra red light through the skin facili- 

 tates photography of superficial veins 

 in the living state. Resolution with this 

 light of relatively long wave length is 

 inferior to that with visible light. 



Injection, see Microinjection. Perfusion 

 of blood vessels and Neutral Red 

 method of staining pancreas by vascular 

 injection. 



Innervation, determination by dissection 

 (Wharton, L. R., Anat. Rcc, 1937, 67, 

 467-475). Place tissue sheets or thin 

 organs on writing paper. Allow to 

 adhere 5-10 min. Place in 1 part gly- 

 cerol, 1 part glacial acetic acid and 6 

 parts 1% aq. chloroal hydrate, 18 hrs. 

 Glj'cerol, 1 part ; Ehrlich's hematoxylin, 

 1 part; and 1% aq. chloral hydrate, 6 

 parts, 24 hrs. or more. If overstained 

 decolorize in first solution or in 1% 

 hydrochloric acid in 70% alcohol. 

 Transfer to glycerol 10 days. Dissect 

 under binocular microscope in fresh 

 glycerol. To make permanent prepara- 

 tions, pass up to 95% alcohol, then 

 through bergamot oil, 2 parts; cedar 

 oil, 1 part; and pure carbolic acid liq- 

 uefied by heat, 1 part, to xylol. Mount 

 in balsam. See Nerve Endings. 



Inoculation is to introduce materials into 

 the body usually disease producing or 

 antigenic. They are in reality injected 

 and we speak of injecting a host of 

 different substances, see in this connec- 

 tion Microinjection, Perfusion and 

 Transplantation. 



Insects. For whole mounts of large insects 

 Stapp, P. and Cumley, R. W., Stain 

 Techn., 1936, 11, 105-106, specify abs. 

 ale, 5-15 days; 95, 85, 70, and 50% each 

 15 min. Ale. 35%, 30 min. Equal 

 parts H2O and H2O2 + trace NH4OH, 

 12-24 hrs. Ale. 35, 50, 85, and 95%, 

 .15 min. each. Abs. ale. 2-3 changes, 

 3 days or more. Toluol, 10-21 days. 

 Pass from thin to thick dammar and 

 mount. Perhaps the simplest method 

 for small insects (fleas, etc.) is simply to 

 drop them in creosote, U.S. P. and after 



24 hrs. to mount them directly in balsam 

 (Fox, 1., Science, 1942, 96, 478). Sec- 

 tioning is facilitated by methods de- 

 signed to soften Chitin, see also Fleas, 

 Ticks. Use of fluorescence microscopy 

 in entomology (Metcalf, R. L. and Pat- 

 ton, R. L., Stain Techn., 1944, 19, 11- 

 27). In making preparations of insect 

 tissues one must of course be on the 

 lookout for infecting organisms. A 

 well illustrated volume, giving many 

 technical details, is that of Paillot, A. 

 L'Infection Chez Les Insectes. Im- 

 primerie de Trdvoux, G. Patissier, 1933, 

 535 pp.. ■ 



Intermitoiic Ceils, see Cell Classification. 



Intestinal Protozoa. 1. Johnson's rapid 

 iron hematoxylin method (Johnson, 

 C. M., Am. J. trop. Med., 1935, 15, 551). 

 Fix thin smears 10 min. in Schaudinn's 

 fixative containing 5-10% glacial acetic 

 acid (37°-45°C). Treat for 5 min. with 

 iodine in 95% alcohol (port wine color). 

 After placing in 70% alcohol for 5 min. 

 rinse in tap water 1-3 min. Mordant 

 in 4% aq. iron alum (purple crystals) 

 for 15 min. Rinse in tap water 1-2 

 min. and stain for 10 min. in 0.5% aq. 

 hematoxylin (10 cc. 5% hematoxylin in 

 95% ale. plus 90 cc. aq. dest.). Differ- 

 entiate in 0.25% aq. iron alum 6-10 min. 

 for flagellates and 12 min. for amoebae. 

 After washing in running water for 3- 

 30 min., dehj'drate in ale, clear in xylol 

 and mount. 



2. Long method of Ileidenhain (Q.M. 

 Gciman in Simmons and Gentzkow, 

 p. 616). Recommended for Balanii- 

 dium coli and for permanent mounts. 

 This is practically the same except for 

 longer mord.anting and staining. See 

 Iron Hematoxylin. 



Intestine. Difference in appearance of wall 

 when contracted and normally distended 

 (Johnson, F. P., Am. J. Anat., 1912-13, 

 14, 235-250). Alterations in human 

 mucosa from absorption of fat and from 

 fasting (Cowdry's Histology, pp. 302- 

 305). Effect of different dehydration 

 and clearing agents on intestine (Ralph, 

 P., Stain Techn., 1938, 13, 9-15). Ros- 

 enberg, L. E., Stain Techn., 1940, 15, 

 53-50 has given an interesting account 

 of postmortem autodigestion. Mingaz- 

 zini phenomenon (Macklin, C. C. and 

 M. T., J. Anat., 1926, 61, 144-150). See 

 Large and Small Intestines. 



Intracellular Phase, see Chloride. 



Intranuclear crystals. Hepatic cells of 

 dogs. Determination of properties 

 (Weatherford, H. L., and Trimble, 

 H. C, Anat. Rec, 1940, 77, 487-502). 



Intranuclear Inclusions, see Nuclear In- 

 clusions. 



lodeosin B, sec Erythrosin, bluish. 



Iodine, detection of: 1. Ionized iodine in 



