KINNEY'S METHOD 



132 



LAMPBLACK 



and pancreas. Results arc sometinaes 

 patchy in the spleen. 



Kleinenberg's fixative. Saturated picric 

 acid in 2% aq. sulphuric acid. Embryos 

 and marine organisms. 



Kolatchew Fluid, see Golgi Apparatus. 



Korff's Fibers of dentin, see Teeth, De- 

 veloping. 



Kossa, see his test for Calcium. 



Krajian's Congo Stain. Elastic fibers (Kra- 

 jian, A. A., Arch. Path., 1934, 18, 378- 

 380). Fix in 10% formalin, 24 hrs. or 

 more. Cut frozen sections. Wash 

 them in tap water. Place in 2% aq. 

 aluminum chloride 5-10 min. Wash and 

 stain 10 min. in 8 cc. 4% Congo red in 

 5% aq. sodium citrate + 2 cc. glycerin 

 C.P. After washing in tap water trans- 

 fer to 1% aq. KI for 10 sec. agitate. 

 After again washing in tap water, stain 

 5-10 min. in :anilin blue, 1.5gm. ; orange 

 G, 2.5 gm.; resorcinol, 3 gm.; phospho- 

 molybdic acid, 1 gm.; aq. dest., 100. 

 Wash carefully in tap water. Blot sec- 

 tions on slides. Dehydrate in absolute 

 alcohol 2 min.; clear in origanum oil; 

 pass through xylol to balsam. Elastic 

 fibers bright red, fibrin dark blue. 



Krause's End-Bulbs. Methylene blue dem- 

 onstration of in skin of forearm (Wed- 

 dell, G., J. Anat., 1940-41, 75, 346-367). 

 See Skin. 



Krause's Membrane. Special technique 

 for, see Dahlgren (McClung, p. 427). 



Kronig's Cement is recommended by Bens- 

 leys (p. 41) for ringing preparations 

 mounted in glycerin jelly or glycerin : 

 7-9 parts colophonium (resin) melted 

 and stirred with 2 parts beeswax. 



Kurloff Bodies are cytoplasmic inclusions 

 which frequently occur in the non-gran- 

 ular leucocytes of guinea pigs. They 

 show particularly well in smears of the 

 spleen, may attain a size equal to that 

 of the nucleus and can be brilliantly 

 colored supra vitally by 1:2000 brilliant 

 cresyl blue in physiological salt solution 

 (Cowdry, E. V. chapter in Rivers'book 

 on Viruses, Baltimore, Williams & Wil- 

 kins, 1928, p. 141). 



Kultsclutzky's Hematoxylin is 1 gm. hema- 

 toxylin dissolved in a little alcohol made 

 up to 100 cc. with 2% aq. acetic acid 

 (Lee, p. 526). 



Lac, a crimson dye oljtaincd from resinous 

 incrustation caused by the insect, Coc- 

 cus lacca, of Siam, Indo-China and 

 Southern India. This dye, introduced 

 into England about 1790 A.D., became 

 an important article of commerce in 

 competition with cochineal of Mexican 

 origin, but before long proved inferior 

 to cochineal and was no longer im- 

 ported. The crimson dyes, Kermes, 

 cochineal and lac have played im- 



portant parts in the history of civiliza- 

 tion (Leggett, W. F., Ancient and 

 Medieval Dyes. Brooklyn: Chemical 

 Publishing Co., Inc., 1944, 95 pp.) 



Lacteals, see Lymphatic Vessels, 



Lactophenol, a fixative for Bilharzial Cer- 

 carlae. See Lactophenol-cotton blue 

 iRchniqao under Fungi. 



Laidlaw's Methods. 1. For inclusion bodies 

 (quoted from Pappenheimer, A. W. and 

 Hawthorne, J. J., Am. J. Path., 1936, 

 12, 625-633, see colored figure, who used 

 it for cytoplasmic inclusions in liver 

 cells). Fix in sat. aq. corrosive sub- 

 limate 100 cc. -f 5% glacial acetic acid 

 or in Zenker's fluid without acetic. 

 Imbed in paraffin, cut sections 3^- Re- 

 move paraffin and pass down to water. 

 Weigert's iron hematoxylin (2%) 5 min. 

 Differentiate in 0.5% acid alcohol. 

 Rinse in tap water, then aq. dest. 1% 

 aq. acid fuchsin 5-15 min. Rinse in 

 aq. dest. Mordant in 1% phospho- 

 molybdic acid 30 sec. Rinse in aq. dest. 

 Differentiate in 0.25% orange G in 70% 

 ale. Dehydrate, clear and mount in 

 balsam . 



2. For silver staining of skin and tu- 

 mors (Laidlaw, G. F., Am. J. Path., 1929, 

 5, 239-247). Fix in Bouin's fluid or in 

 10% neutral formalin for 3 days. (To 

 make the Bouin's fluid he uses, add 100 

 cc. commercial formalin and 20 cc. glacial 

 acetic acid to 300 cc. tap water and satu- 

 rate with picric acid). Fix paraffin 

 sections to slides by Masson's Gelatin 

 Glue. Wash Bouin sections for 20 min. 

 in running water, and formalin ones for 

 5 min. 1% ale. iodine, 3min., rinse in tap 

 water. 5% aq. hypo (sodium thiosul- 

 phate), 3 min., rinse in tap water. 

 §% aq. potassium permanganate 3 min., 

 rinse in tap water, 5% oxalic acid, 5 min. 

 Wash in running water, 10 min. Aq. 

 dest. 3 changes in 5-10 min. to clean 

 before adding silver. Heat stock Lith- 

 ium Silver solution to 50 °C. and stain 

 in oven for 5 min. Pour aq. dest. over 

 both sides of slides. Flood sections fre- 

 quently for 3 min. with 1% formalin in 

 tap Vv'ater. Again rinse both sides of 

 slides with aq. dest. 1:500 yellow gold 

 chloride in aq. dest. in Coplin jar at 

 room temperature, 10 min. Rinse both 

 sides with aq. dest. Pour on 5% oxalic 

 acid 10 min. Rinse in aq. dest. Pour 

 on 5% hypo changing as often as it be- 

 comes turbid, 10 min. Wash in running 

 water. Counterstain if desired. De- 

 hydrate, clear and mount in usual way. 

 Reticulum, black threads; collagen red- 

 dish purple. 



Lake Ponceau, see Ponceau 211. 



Lampblack. A colloidal suspension of lamp- 

 black is an excellent substance to inject 

 intravenously to demonstrate phago- 



