STARCH GRAINS 



231 



SUBMAXILLARY GLANDS 



nin, 20min. Whas in aq. dest. and stain 

 in 1% aq. toluidin blue, gentian violet 

 or methyl green, 5-10 min. Milovidov, 

 (P. F., Arch. d'Anat. Micr., 1928, 24, 

 8-18). Differentiate in 95% ale. dehy- 

 drate in abs. ale, clear in xylol and 

 mount. Mitochondria red, starch blue, 

 violet or green. Well shown in an 

 excellent colored plate. Armed with 

 illustrations showing the distinctive 

 structural features of starch granules 

 from many species of plants it is ordi- 

 narily a simple matter by direct micro- 

 scopic examination to identify a given 

 sample of starch (Schneider, A., The 

 Microbiology and Microanalysis of 

 Foods. Philadelphia: P. Blakiston's 

 Son & Co., 1920, 262 pp.). 



Starch Paste, as substitute for albumin- 

 glycerin mixture in mounting paraffin 

 sections. Mix thoroughly 1 gm. pow- 

 dered starch in 10 cc. cold water. Pour 

 into 20 cc. boiling water. Add 2 drops 

 dilute HCl and boil 5 min. constantly 

 stirring to free opalescent sol. from 

 lumps of starch. Add crystal of thymol 

 after paste has cooled. Use as the albu- 

 min mixture. Has advantages in stain- 

 ing techniques as it is unaffected by 

 dyes, gives a very light background 

 especially in silver preparations; it is 

 easily made, and sections adhere firmly 

 to slides. R. Spoerri, Science, 1939, 

 90, 260, see also McDowell, A. M., and 

 Vassos, A. A. Jr., Arch. Path., 1940, 29, 

 432-434. 



Steel Gray, see nigrosine, water soluble. 



Stereocilia of ductus epididymis are not true 

 cilia. For technique and discussion, 

 see Lucas A. ,M., in Cowdry's Special 

 Cytology, 1932 1, 409-474. 



Sternberg Cells, see Reed-Sternberg Cells. 



Stomach, secretory cells of. Use Mucicar- 

 mine or Mucihematein for surface 

 epithelial cells and neck chief cells; 

 Bensley's Neutral Gentian for body 

 chief cells and any combination of dyes 

 including a strongly "acid" stain like 

 eosin for the parietal cells, all after Ben- 

 sley's alcoholic chrome neblimate fixa- 

 tion. The parietal cells can be sharply 

 stained by supravital intravascular in- 

 jection with Neutral red or Naphthol 

 Blue R. The canaliculi of the parietal 

 cells can be impregnated with silver by a 

 modified Golgi method (Plenk, H., von 

 Mollendor'f Handb. d. Mikr. Anat. d. 

 Menschen. 1932, 5, (2), 235-402). To 

 observe the cytological changes after 

 discharge of strongly acid gastric juice 

 and of juice rich in pepsin inject hist- 

 amine and stimulate the vagus respec- 

 tively (Bowie, D. J., and Voneberg, A. 

 M., Quart. J. Exper. Physiol., 1935 

 25, 247-257). For mitochondria inject 

 Janus Green intravascularly or fix in 

 Regaud's fluid, mordant in potassium 



bichromate and stain with Anilin- 

 Fuchsin Methyl Green. See localiza- 

 tion of Pepsin. 



Stools, see Feces. 



Storage of specimens whether microscopic 

 slides, paraffin or celloidin blocks or 

 simply in preservative fluids should be 

 systematic in all laboratories. Every 

 specimen coming in for examination 

 should be given an accession number 

 and the data about it should be inscribed 

 in a book. A book is better than a series 

 of cards because cards can be removed 

 by irresponsible persons and lost. The 

 number, and other necessary informa- 

 tion, should be written on the slide with 

 a diamond pencil. This is usually done 

 in pathological laboratories where there 

 is much routine to be attended to. It is 

 equally important in other laboratories 

 devoted primarily to teaching and re- 

 search even when a number of inde- 

 pendent investigators are involved. 

 System pays; lack of a unified system 

 serving several people means loss and 

 waste of valuable material. 



Strength, see Tensile. 



Striated Cuticular Border of intestinal epi- 

 thelial cells is frequently confused with 

 cilia, see Lucas, A. M., in Cowdry's 

 Special Cytology, 1932, 1, 409-474. 



Striated Muscle, glycogen distribution 

 (Gendre, H., Bull. d'Hist. Appl., 1938, 

 15, 265-276). Effect of different dehy- 

 dration and clearing agents (Ralph, P., 

 Stain Techn., 1938, 13, 8-15). Methods 

 for study of wave mechanics in living 

 state (Carey, E. J., Zeit, W. and Masso- 

 pust, L., Am. J. Anat., 1942, 70, 119-133. 



Styrax, a very highly refractile mounting 

 medium seldom employed in histology 

 (Lee, p. 228). 



Subcutaneous Tissue spreads. Making 

 (McClung's Microscopical Technique 

 p. 336). 



Sublimate Acetic is a fixative of which the 

 usual composition is 95 parts sat. aq. 

 mercuric chloride plus 5 parts glacial 

 acetic acid. See Laidlaw's method for 

 inclusion bodies. When the saturated 

 solution of mercuric chloride is made in 

 95% alcohol the fixative should be called 

 Sublimate Alcohol Acetic. See Mer- 

 curic Chloride. 



Submaxillary Glands. These can be nicely 

 stained by the supravital methods em- 

 ployed for the Pancreas. Stains for 

 Zymogen and for Mucus are useful. The 

 duct cells are the principal sites of 

 action of the salivary glana virus when 

 this plays an inapparent role. The 

 tremendously enlarged duct cells pro- 

 vided with Nuclear Inclusions are often 

 seen in the guinea pig's submaxillary 

 and in several other species, see Cowdry , 

 E. V. and Scott, G. H., Am. J. Path., 

 1935, 11, 647-657. 



