pound of methyl green. For the purposes for which this stain was 

 desired this batch may be satisfactory; but the user can hardly 

 count on its being the same as it was when imported nor on its 

 remaining unchanged until used up. 



IODINE GREEN 



Synonym: Hoffmann s green. 



c. I. NO. 686 



This dye is closely related to methyl green, the generally accept- 

 ed formula being: 



CHj CI 



\l 

 N_ 



/ " 

 CH, 



CI CHj 



1/ 

 /-\_N-CH3 



/V_/ \ 

 / CH3 



.=C CHj 



./ \ ^ / 



\ CH3 



CHj 



{A basic dye.) 



Iodine green is a nuclear or chromatin stain which has selective 

 properties that make it of value in certain special procedures. It 

 is used by Ciaccio for nervous tissue in combination with acid 

 fuchsin and picric acid; and by Lefas as a blood stain in combina- 

 tion with acid fuchsin. It is used by Zimmermann with basic 

 fuchsin for staining chromatin in plant tissue; while together with 

 acid fuchsin it is occasionally used by botanists for staining lignified 

 xylem. It is also used for staining mucin and amyloid, having the 

 property of giving the latter a red instead of a green color. 



SPIRIT BLUE 



C. I. NO. 689 



Synonyms: Gentian blue. Anilin blue, alcohol soluble. Night blue. 



Lyons blue. Paris blue. 



This is a mixture of di-phenyl rosanilin 



CH, 



71 



