10 



INTRODUCTION TO PHASE MICROSCOPY 



At the focal plane of the eyepiece the direct light has spread over the 

 field of view, and the deviated light, now converged, interferes with the 



Undeviated wave 



Focal plane 

 of eyepiece 



Deviated wave , 

 Dielectric layer 



Diffraction plate; 



back focal plane 



of objective 



Objective 



Specimen 



Condenser 



Condenser diaphragm 



Fig. 1.5. Diagram of light path through a phase microscope. 



direct light to form a light or dark image of the corresponding point in 

 the specimen. 



Referring again to Figs. 1.3 and 1.4, in order to obtain a maximum 

 difference between the light in the image of the particle P and that in its 

 background S, we should make the amplitudes of the waves S and D 



