194 



PHASE MICROSCOPY IN BIOLOGY AND MEDICINE 



2. TECHNIC 



The ability of the phase microscope to reveal detail in unstained, 

 living cells has placed emphasis on preparation methods which do not 

 alter or damage the cells and tissues. Many of the technics described 

 in the previous chapter are appropriate and will not be repeated here. 

 Some cells and organisms will remain from a few hours to several days 

 in a sealed mount with no apparent change, whereas others require an 

 effectively constant environment for survival. The latter may be put 

 in mounts through which nutrients may be flowed and excretion products 

 washed out (Tinsley, 1938). Moment (1944) recommends polyvinyl 

 alcohol for quieting protozoa and other small organisms. 



Tal.le V.l 



A. Biological materials 



Ut 



°C Reference 



15 Porter (1947) 

 . . Porter (1947) 



Calkins and Summers (1941) 

 . . Heilbrunn (1937) 



. . Heilbrunn (1937) 

 . . Heilbrunn (1937) 

 . . Frederikse (1933a, b) 



Calkins and Summers (1941) 



Calkins and Summers (1941) 

 Calkins and Summers (1941) 



Calkins and Summers (1941) 

 Calkins and Summers (1941) 



. . Heilbrunn (1937) 



. . Schmitt (1944) 



. . Schmitt (1944) 



18 Schmitt (1944) 



. . Waugh and Schmitt (1940) 



. . Waugh and Schmitt (1940) 



. . Waugh and Schmitt (1944) 



. . Waugh and Schmitt (1944) 



. . Thorell (1947) 



17 0.0013/°C, du Noiiy (1929) 



37 



Thorell (1947) 

 Antonio (1949) 



Pantin (1946) 



American Optical Co. Chart 

 American Optical Co. Chart 



