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PART I. BASIC PROCEDURES 



Chapter 1. Fixatioyi 3 



Chemicals Commonly Used in Fixing Solutions ... 5 



Maceration (a prefixation process) 10 



Fixing the Tissue 11 



Washing the Tissue 12 



Fixing Solutions and Their Uses 13 



Fixation by Perfusion 22 



Post-fixation Treatments 23 



Decalcification 24 



Chapter 2. Dehydration 30 



Preparation for Embedding 30 



Special Treatment for Small, Colorless Tissues ... 32 



Chapter 3. Clearing, Infiltrating, and Embedding for Paraffin 



Method 33 



Clearing 33 



Infiltration with Paraffin 35 



Embedding with Paraffin 36 



Embedding Cellular Contents of Body Fluids .... 38 



Dehydration and Clearing Combinations 39 



Timing Schedule 41 



Automatic Tissue Processors 42 



Chapter 4. Microtomes and Microtome Knives 43 



Microtomes 43 



Microtome Knives 44 



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