Fixing Solutions and their Uses 15 



HOLLANDE bouin's FIXATIVE (Roiueis, 1948): 8 hours-8 days. 



copper acetate 2.5 gm. 



picric acid crystals 4.0 gm. 



formalin 10.0 ml. 



distilled water 100.0 ml. 



glacial acetic acid 1.5 ml. 



Dissolve copper acetate in water ^vithollt heat; add picric acid slowly 

 with stirring. When dissolved, filter, and add formalin and acetic 

 acid. Keeps indefinitely. Hartz (1947) recommends this for fixation of 

 calcified areas as in lymph nodes or fat necrosis. It is a good general 

 fixative. Wash for several hours in 2 or 3 changes of distilled water. 



orth's fixative (Gatenby, 1950 and Galigher, 1934): 12 hours at room 

 temperature, 3 hours at 37 °C. 



formalin 10.0 ml. 



potassium dichromate 2.5 gm. 



sodium sulfate 1 .0 gm. 



distilled water 100.0 ml. 



Mix fresh. A good routine fixative, also for glycogen and fat. Wash 

 in running water overnight. 



Lillie's {1954B) variation: 



2.5% potassium dichromate 100.0 ml. 



formalin 10.0 ml. 



stieve's fixative (Romeis, 1948): 24 hours. 



mercuric chloride, saturated aqueous 7(i.O ml. 



formalin 20.0 ml. 



glacial acetic acid 4.0 ml. 



Similar in effect to Susa's below, but simpler to prepare. Penetrates 

 rapidly, good for large pieces. Time not critical. Go directly to 70% 

 alcohol. Post-treat for mercuric chloride. 



susa's fixative (Romeis, 1948): 24 hours. 



mercuric chloride saturated in 0.6% NaCl .... 50.0 ml. 



trichloracetic acid 2.0 gm. 



glacial acetic acid 4.0 ml. 



formalin 20.0 ml. 



distilled water 30.0 ml. 



