Fixing Solutions and their Uses 17 



Formula B: 



glacial acetic acid 10.0 ml. 



absolute ethyl alcohol 60.0 ml. 



chloroform 30.0 ml. 



Chloroform is said to make action more rapid. Important fixative for 

 glycogen and Nissl substance, but dissolves most other cytoplasmic 

 elements. Wash 2-3 hours in absolute alcohol to remove chloroform, 

 particularly if embedding in nitrocellulose. 



CARNOY-LEBRUN FIXATIVE {LHUe, 19548): 3-6 hours 



absolute ethyl alcohol 15.0 ml. 



glacial acetic acid 15.0 ml. 



chloroform 15.0 ml. 



mercuric chloride crystals 4.0 gm. 



Lee of The Microtoruist's Vade Mecum fame considers this a very fine 

 fixative. It does not keep; mix fresh. Penetrates rapidly. Wash well in 

 alcohol to remove chloroform. 



CHAMPY FIXATIVE (Gateuby, 1930): 12 hours. 



potassum dichromate, 3% aqueous (3 gm./lOO 



ml. water) 7.0 ml. 



chromic acid, 1% aqueous (1 gm./lOO ml. wa- 

 ter) 7.0 ml. 



osmic acid, 2% aqueous (2 gm./lOO ml. water) 4.0 ml. 



Prepare immediately before use. Good for cytological detail, mito 

 chondria, lipids, etc. Penetrates poorly; use only small pieces of tis- 

 sue. Wash in running water overnight. 



DAFANO FIXATIVE (Romels, 1948): 12-24 hours. 



cobalt nitrate 1.0 gm. 



formalin . .' 15.0 ml. 



distilled water 100.0 ml. 



Wash in water. Good for Golgi apparatus. 



flemming's fixatives; 12-24 hours. 

 A. Strong solution 



chromic acid, 1% aqueous (1 gm./lOO ml. wa- 

 ter) 15.0 ml. 



osmic acid, 2% aqueous (2 gm./lOO ml. water) . 4.0 ml. 



glacial acetic acid 1.0 ml. 



