18 ' Fixation (chap. 1) 



Mix just before using. Acts slowly: use small pieces with no blood on 

 outside. Wash in running water for 24 hours. 



B. Modification for cytoplasmic study. High acetic content of A 

 solution makes it poor for cytoplasm fixation. 



chromic acid, 1% aqueous (1 gm./lOO ml. wa- 

 ter) 15.0 ml. 



osmic acid, 2% aqueous (2 gm./lOO ml. water) . 4.0 ml. 



glacial acetic acid 2-3 drops 



C. Weak solution 



chromic acid, 1% aqueous (1 gm./lOO ml. wa- 

 ter) 25.0 ml. 



osmic acid, 2% aqueous (2 gm./lOO ml. water) . 5.0 ml. 



acetic acid, 1% aqueous (1 ml./99 ml. water) . . 10.0 ml. 



distilled water 60.0 ml. 



Flemming's solutions are good for mitotic figures (not suitable for 

 general work), btit they penetrate poorly, harden excessively, blacken 

 material, and interfere with action of many stains. The weak solution 

 is a fine fixative for minute and delicate objects. For dense tissues use 

 the strong solution. Iron hematoxylin is excellent following these 

 fixatives. 



FORMOL-ALCOHOL (Tcllyesniczky) (Lillie, 1934 B): 1-24 hours 



70% ethyl alcohol 100.0 ml. 



formalin 5.0 ml. 



glacial acetic acid 5.0 ml. 



Good for insects and Crustacea. Widely used by Botanists. Transfer 

 to 85% alcohol. 



gendre's fluid {Lillie, 1954B): 1-4 hours, 25°C. 



95% ethyl alcohol saturated with picric acid . . . 80.0 ml. 



formalin 15.0 ml. 



glacial acetic acid 5.0 ml. 



Good for glycogen. Wash in several changes of 80% alcohol. 



gilson's fixative (Gatenby, 1930): 24 hours; may be left several days. 



nitric acid, concentrated 15.0 ml. 



glacial acetic acid 4.0 ml. 



mercuric chloride crystals 20.0 gm. 



60% ethyl alcohol 100.0 ml. 



distilled water 880.0 ml. 



