Rapid Staining Methods HO 



Reiner (1953) Method (Uses Stock Giemsa) 



1. Add 1-2 drops stock Giemsa to section on slide: 5-10 seconds. 



2. Float section off into dish of acidulated water (3 drops glacial acetic 

 acid to 500 ml. distilled water). Allow to differentiate until dense 

 clouds of color cease to stream off: 10-15 seconds. 



3. Transfer to water and remount on clean slide. 



4. Remove excess water, mount in glycerine jelly. 



