78 Nitrocellulose Method (chap. 7) 



c. Pour clove oil over it: 5-20 minutes. 



(l. 95% alcohol, 3 changes: 5-10 minutes. 



e. Absolute alcohol-ether, 2 changes, to dissolve nitrocellulose. 



/. 70% alcohol, etc., to stain. 



Method 2 (Lewis, 1945) 



a. Rub on slide one drop Haupt's fixative (page 413). 



b. Transfer section on cigarette paper to slide. 



c. Blot, and firmly press with filter paper. 



d. Roll off cigarette paper rapidly; section must not dry. 



e. Place immediately in absolute alcohol-ether luitil nitrocellulose 

 is dissolved: 10-20 minutes. 



/. Remove to 70% alcohol, etc., to stain. 



Method 3 



a. Float sections onto slide and blot firmly with filter paper. 



b. Dip slide into 0.5% nitrocellulose for few seconds; drain and wipe 

 back of slide clean. 



c. Harden film in chloroform: 5-10 minutes. 



d. 95%, 70% alcohol, etc., to stain. 



Method 4 (Culling, 1957) 



a. Transfer section to 95% alcohol: 1-2 minutes. 



b. Float on slide, drain for few minutes, blot lightly to flatten section, 

 but do not dry. 



c. Pour ether vapor over sections — only vapor, not liquid. This par- 

 tially dissolves nitrocellulose and causes it to adhere to slide. 



d. Place slide in 80% alcohol: 5 minutes, to harden nitrocellulose. 



e. Running water: 10 minutes; stain. 



Method 5 — Serial Sections 



a. Arrange sections on knife from left to right. 



b. Lay cigarette paper on sections and saturate with 70% alcohol. 



c. With quick sweeping movement, pull paper off knife and carry to 

 slide. Smooth with brush. 



d. Place filter paper on top and press flat, until much of alcohol is 

 absorbed. 



