Mounting Before Staining 79 



e. Jerk off paper and cover with clove oil: 3-5 minutes. 

 /. Submerge in 95% alcohol. 

 g. 70% alcohol, etc., to stain. 



Method 6 — Serial Sections (Williams, 1957) 



a. Coat clean slides by dipping in warm solution ot 1% gelatine. 

 Allow to drain and dry in vertical position. 



b. Lay sections on blotting paper moistened with 70% alcohol. Keep 

 covered to prevent drying. 



c. Pick up sections, one at a time, dip in 70% alcohol and arrange in 

 order on coated slides. 



d. Blot sections with dry filter paper, flattening and pressing them 

 into contact with gelatine. 



e. Place in coplin jar containing 2-3 cc. formalin. Do not allow fluid 

 to come in contact Avith sections, it may cause them to float off 

 slide. 



/. Allows slides to remain tightly covered, 2-3 hotirs, room tempera- 

 ture. 

 g. 70% alcohol, etc., to stain. 



Method 7 — Serial Sections (Wetmore, 1932) 



a. Place on slide in order cut. Keep slides moist with 95% alcohol. 



b. Blot off excess alcohol ^vith filter paper. 



c. Paint in spaces between sections Avith 2-4% nitrocellulose and 

 allow' to dry. Thin sections require 2%, thicker ones 4%. Keep as 

 little as possible from getting on sections or margins may ctirl. 



d. Place slides and sections in 95% alcohol plus a little chloroform to 

 harden, approximately 30 minutes. 



e. With scalpel or section lifter, remove sheet of sections from slide 

 and store in 95% alcohol until ready to stain. 



The author hesitates to recommend any of the above methods over 

 others. Adopt the one that cooperates the most effectively. 



