82 Specialized Embedding Technics (chap. 8) 



Sections while floating on this sohition are picked up on a slide and 

 allowed to dry. 



Wade (1952) Method 



Wade prefers to albiimenize slides with a thin coat of Mayer's egg 

 albumen (page 412). Dry overnight or three hours or longer in an oven. 

 Floating ribbons intact and Avithoiit wrinkles still remains a problem. 

 He suggests that the addition of a wetting agent, Tiirgitol 7 (0.005% in 

 distilled Avater), will reduce surface tension effects. Add 10% of 15-fO 

 Carbowax to reduce shrinkage while sections are drying. 



Giovacchini (1958) Method 



Giovacchini thinly smears slides with the following solution: Dissolve 

 15 grams of gelatine in 55.0 ml. distilled water by heating. Add 50.0 ml. 

 glycerine and 0.5 gram phenol. Place sections on slides and place on 

 warming plate at 58-60°C for 15 minutes. Transfer to drying oven, 

 58°C, for 24 hours. Ready for staining. 



Jones et al. (1959) Method 



Jones and his coworkers float their sections on: 



diethylene glycol 100.0 ml. 



formalin 7.0 ml. 



carbowax 1.0 ml. 



distilled water 400.0 ml. 



In this method more spreading results from increasing the propor- 

 tion of water. If the tissues overexpand, add a small amoimt of Zephiram 

 chloride concentrate (5-10 drops to 500.0 ml. flotation solution); this 

 reduces surface tension and prevents air bubbles from being trapped 

 between tissue and solution surface. Mount on albimienized slides and 

 dry. 



Pearse (1953) Method 



This method combines features of the above two methods. Pearse 

 smears with Giovacchini's fluid and mounts the sections with the di- 

 ethylene glycol mixture. 



Zugibe et al. (1958) Method 



This method cuts off a section from the ribbon ^vith a razor blade. 

 One edge of the section adheres to the blade. Touch the loose section 

 edge against a slide coated with a flotation fluid and draw the rest of 

 the section onto the slide. 



