specialized Microscopy 103 



by secondary fluorescence induced by fluorochromes, while the latter 

 records specific absorption of the ultraviolet light by certain structures 

 in the specimen. 



USES FOR FLUOROCHROMES 



Granules of islets of Langerhans: Hartrojt {19'>1)\ mucin: Hicks and 

 Matthaei (193S); blood vessels and lymphatics: Schlegel {1949); muco- 

 polysaccharides: Kityper (19^7); bone marrow: Werth (19'>3); amyloid 

 and connective tisstie: Vassar (uid Culling {19^9); nerve cells: Zeiger 

 et at. {1951); fat: Metcalf and Pat ton {1944); nucleic acids, nucleopro- 

 teins: Armstrong {1956), dcBruyn et al. {1953); cancer: Vinegar {1916), 

 von Bertalanffy and von Bertalanffy {I960), and Umiker and Pickle 

 {I960); alkaline phosphatase: Burstone {I960). This bibliography will 

 easily lead to many others. 



Electron Microscopy 



In the electron microscope magnets focus an illuminating beam of 

 electrons onto the specimen. The scattering of the electrons by the 

 specimen forms shadows that can be photographed on film. Discussion 

 concerning this method is found on page 397. 



Microscopy References 



Barer (1956, 1959); Beck (1938); Belling (1930); Bennett (1950); 

 Davis (1958); Dempster (1944 A and B); Engstrom (1956, 1959); Gage 

 (1943); Ham (1957): McClung-Jones (1950); Munz and Charipper 

 (1943); Needham (1958); Nurnberger (1955); Oster (1955); Popper and 

 Szanto (1950); Richards (1954); Ruch (1955); Scott (1955); Shillaber 

 (1944); Vickers (1956) and AValker (1958). 



