122 Staining Procedures (chap. 11) 



Dissolve gelatine in boiling water, add glycerol. After mixing add 

 chrome alum solution and phenol. Liquefies in 15 minutes at 65°C. 

 May transfer from glycerol or formalin directly. Hardens to form 

 permanent mount. 



Abopon 



This mounting medium is water miscible and can replace glycerol 

 jelly in several special staining techniques. It can be purchased from the 

 Glyco Chemicals Company, Williamsport, Pennsylvania, in the form of 

 lumps or solution. Dissolve the solid form, approximately 50 gm., in 

 25 ml. distilled water. The fluid form usually is too thick and is diluted 

 ^vith distilled water. 



Diaphane 



This medium is available in two forms, colorless (r.i., 1.4777) and 

 green (r.i., 1.4792). Slides can be mounted directly from absolute, 95% 

 or 70% alcohol. It and its solvent can be purchased from the Will Cor- 

 poration. 



Fluorescent Mounting Medium (Rodriquez and Deinhardt, I960) 



Elvanol 51-05 (DuPont. See note, p. 120) 20.0 gm. 



0.1 4M Sodium chloride buffered with 0.0 IM 



KH0PO4 - NaoHP04- I2H2O, pH 7.2 80.0 ml. 



add glycerol 40.0 ml. 



Asfitate another 16 hours 



'O' 



Remove undissolved particles of Elvanol by centrifuging, 12,000 rpm, 

 15 minutes. The pH should be between 6 and 7. 



Aqueous Mounting Technics 



Aqueous mounts — sections or whole mounts — are removed from the 

 water, placed on a slide and covered with a drop of moimting medium. 

 The cover glass, held in a horizontal position, is placed directly on the 

 medium. Do not drop it from a slanted position. By the former method, 

 the object can be kept centered and not carried to one side. In many 

 cases it is not necessary to press the cover glass into place; its own weight 

 is sufficient. The sections or objects are not attached to the slides and 

 too much pressure may result in disarranged and broken material. 



