Chapter J 2 



Hematoxylin 

 Staining 



SUBSTITUTES AND COUNTERSTAINS 



Single Solutions 



Delafield's Hematoxylin (carlton, 1947) 



Dissolve 4 gm. hematoxylin in 25 ml. absolute ethyl alcohol. Mix 

 gradually into 400 ml. ammonia alum, Al2(S04)3(NH4)2S04-24H20, 

 saturated aqueous (approximately 1 part alum to 1 1 parts distilled wa- 

 ter). Leave exposed to light in a flask with a cotton phig for 3-5 days. 

 Filter. Add to the filtrate, 100 ml. glycerine and 100 ml. methyl alcohol. 

 Ripen for at least 6 weeks. The ripened solution will keep for years in 

 a stoppered bottle. 



Ehrlich's Hematoxylin (gurr, 1956) 



hematoxylin 2.0 gm. 



ammonia alum, Alo(S04)3(NH4)oS04-24HoO .. 3.0 gm. 



alcohol, methyl or ethyl 100.0 ml. 



glycerol 100.0 ml. 



distilled water 100.0 ml. 



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