single Soliilions 125 



Ripens in 6-8 weeks, or may be ripened for immediate use with 2.4 

 om. sodium iodate. 



Add 10 ml. glacial acetic acid. Keeps for years. 



Harris' Hematoxylin (mallory, 1944) 



Dissolve 1.0 gm. hematoxylin in 10 ml. ethyl alcohol. Dissolve 20 

 gm. potassium or ammonia aknii, Al2(S04)3K2S04 -241^20 or Al2(S04)3 

 (NH4)2S04-24H20, in 200 ml. water and boil. Add hematoxylin and 

 boil I minute. Add 0.5 gm. mercuric oxide. Cool rapidly. Add few 

 drops of glacial acetic acid to keep away metallic luster and brighten 

 nuclear structure. Does not keep longer than a month or two. 



Mayer's Hematoxylin (mallory, 1944) 



Add 1 gTn. hematoxylin to 1 liter distilled water. Heat gently and 

 add 2 gm. sodium iodate and 50 gm. potassium alum, Al2(S04)3K2S04 • 

 24H2O. Heat until dissolved and add 1 gm. citric acid and 50 gm. 

 chloral hydrate. Preferably ripen for 6-8 weeks, but can be used within 

 1-2 weeks. 



Papamiltiades Hematoxylin (1953) 



hematoxylin, 1% aqueous 100.0 ml. 



aluminum sulfate, 5% aqueous 50.0 ml. 



zinc sulfate, 5% aqueous 25.0 ml. 



potassium iodide, 4% aqueous 25.0 ml. 



glacial acetic acid 8.0 ml. 



glycerol 25.0 ml. 



Ready for immediate use; keeps approximately 2 months. 



Phosphotungstic Acid Hematoxylin (lieb, 1948) 



phosphotungstic acid 10.0 gm. 



hematoxylin 0.05 gm. 



red mercuric oxide 0.025-0.05 gm. 



hydrogen peroxide 2.0 ml. 



distilled water 500.0 ml. 



Dissolve hematoxylin in a little water with heat. Dissolve phospho- 

 tungstic acid in rest of water with heat; add hematoxylin solution. 

 Bring to boil. Cautiously add mercuric oxide, and remove from 

 flame. Cool. Add hydrogen peroxide. Ready for use in 5-7 days; 

 should be brownish red in color. 



