126 Hematoxylin Sfaijiing (chap. 12) 



Double Solutions 



Weigert's Iron Hematoxylin (mallory, 1944) 



SOLUTION A. 



iron chloride, FeCls, 29% aqueous 4.0 ml. 



distilled water 95.0 ml. 



hydrochloric acid (sp.gr. 1.88-1.92, 37-38% HCl) 1.0 ml. 



SOLUTION B. 



hematoxylin 1.0 gm. 



95% ethyl alcohol 100.0 ml. 



Mix equal parts of A and B. This is best prepared each time, but will 

 keep for 7-8 days once mixed. 



Groat's Variation of Weigert's Hematoxylin (1949) (a single solution) 



distilled water 50.0 ml. 



sulfuric acid (sp.gr. 1.84, 94% HoSOj) 0.8 ml. 



ferric alum, Fe2(S04)3(NH4)2S04-24H20 1.0 gm. 



957o ethyl alcohol 50.0 ml. 



hematoxylin 0.5 gm. 



Mix in order given at room temperature. Filter. Groat recommends 

 his solution as better than Weigert's; it stains well within 10 minutes. 



Lillie's Variation of Weigert's Hematoxylin (lillie and earle, 1939A) 



SOLUTION A. 



ferric alum, Feo(S04)3(NH4)2S04-24H20 20.0 gm. 



distilled water 200.0 ml. 



solution b. 



hematoxylin 2.0 gm. 



absolute methyl alcohol 60.0 ml. 



glycerol 60.0 ml. 



Janssen's Hematoxylin (lillie and earle, 1939A) 



solution a. 



ferric alum, Fe2(S04)3(NH4)2S04-24H20 15.0 gm. 



ferrous sulfate, FeS04-H20 15.0 gm. 



distilled water 100.0 ml. 



