Double Solutions 127 



SOLUTION B. 



hematoxylin 1 .0 gm. 



95% ethyl alcohol 50.0 ml. 



glycerol 50.0 ml. 



Mix A and B in equal amoimts. Can be kept unchanged for several 

 months, but if solution has turned brown, it is no longer usable. 

 Keeps better than Weigert's. 



Hansen's Iron Trioxyhaematin (pantin, 1946) 



SOLUTKJN A. 



ferric alum, Fe2(S04)3(NH4)oS04-24HoO 10.0 gm. 



ammonium sulfate 1.4 gm. 



distilled water 150.0 ml. 



SOLUTION B. 



hematoxylin 1.6 gm. 



distilled water 75.0 ml. 



Dissolve both solutions with gentle heat. Cool. Pour solution 5 in a 

 porcelain evaporating dish. Add A, stirring constantly. Heat slowly 

 without stirring just to boiling point. Cool rapidly by floating dish 

 on cold water. A deep violet color turns brown. Filter into stoppered 

 bottle with little air space above solution. Keeps 6-8 months, but if 

 it develops a green sheen it is unsatisfactory. Can be used progres- 

 sively or regressively. 



Double Solutions, Never Mixed Before Use 



Heidenhain's Iron Hematoxylin 



SOLUTION A. 



ferric alum, Feo(S04)3(NH4).S04 -24^.0 4.0 gm. 



distilled water 100.0 ml. 



Keep in refrigerator to prevent precipitation on sides of bottle. 



SOLUTION B. 



hematoxylin 10.0 gm. 



95% ethyl alcohol 100.0 ml. 



