Other Acceptable Counterstains 129 



Hematein (Hemalum) (kornhauser, 1930) 



hematein 0.5 gm. 



95% ethyl alcohol 10.0 ml. 



Grind hematein with alcohol in glass mortar; 

 Add to potassium alum, Al2(S04)3KoS04-24HoO 



5% aqueous 500.0 ml. 



Immediately ready for use. 



Counterstains for Hematoxylin, 

 Gallocyanin and Hematein 



Eosin 



eosin Y, C.I. 45380 1.0 gm. 



70% ethyl alcohol 1000.0 ml. 



glacial acetic acid 5.0 ml. 



Dilute with equal volume of 70% alcohol for use aud add 2-3 drops 

 of acetic acid. 



Eosin (putt's, 1948) 



eosin Y, C.I. 45380 1.0 gm. 



potassium dichromate 0.5 gm. 



saturated aqueous picric acid 10.0 ml. 



absolute ethyl alcohol 10.0 ml. 



distilled water 80.0 ml. 



glacial acetic acid (optional) 1 drop 



Eosin-Orange G 



\% eosin Y, C.I. 45380, in 95%, ethyl alcohol . 10.0 ml. 



orange G, C.I. 16230, saturated solution in 

 95% ethyl alcohol (approximately 0.5 gm. per 

 100 ml 5.0 ml. 



95% ethyl alcohol 45.0 ml. 



Other Acceptable Counterstains 



1. Acid fuchsin, C.I. 42685, 5%, aqueous (slightly acidified improves 

 staiu). If overstained, riuse with tap ^vater. 



