130 Hematoxylin Staining (chap. 12) 



2. Orange G, C.I. 16230, saturated in 957^ ethyl alcohol. 



3. Van Gieson or substitute, page 165. 



4. Bordeaux red, C.I. 16180, 1% aqueous. 



5. Biebrich scarlet, C.I. 26905, 1% aqueous, a good counterstain. 



6. Additional "eosins" 



a. Eosin }', C.I. 45380, 0.1-0.5% in 957o ethyl alcohol. 



b. Erythrosin B, C.I. 45430, 0.1-0.5% in 95% ethyl alcohol. 



c. Phloxine B, C.I. 45410, 0.5% aqueous, plus a few drops of acetic 

 acid, page 229. 



7. Congo red, C.I. 22120, 0.5% aqueous. 



8. Light green SF, yellowish, C.I. 42095, 0.2-0.3%o in 95% ethyl alco- 

 hol. 



9. Aniline blue W.S., C.I. 42780, pages 147, 149, 150, 160. 



10. Fast green FCF, C.I. 42053, similar to light green or aniline blue. 



Hematoxylin Staining Procedures 

 Delafield's (or Harris) Hematoxylin 



Progressive Method 

 fixation: any general fixative or one specific for nuclear detail. 



solutions: 



Hematoxylin, page 124. 

 Counterstain, page 129. 



procedure: The slides are passed through a "down" series of jars, a 

 process often termed running down slides to water (or hydration) be- 

 cause a series of alcohols of decreasing strenoth is used. (From left to 

 right, top row of Fig. 26) Never at any time during this procedure 

 allow the slides to dry, 



1, xylene (or toluene) 2-3 minutes or longer. 



(2 changes may prove profitable to insure complete removal of 

 paraffin.) 



2. absolute alcohol 2-3 minutes or longer. 



3. 95% alcohol 2-3 minutes or longer. 



4. 70% alcohol 2-3 minutes or longer. 



If mercuric chloride was absent from fixative, skip steps 5 through 

 7 and proceed into step 8. 



