Hematoxylin Stai?ii)ig Procedures 



131 



RUNNING WATER 



t / t / \ ( 



C^—(^- 





-[lUGOL'sJ ( ^^Q J f HEMA j fsCOTT'sj 



Figure 26. A suggested arrangement of staining jars. An alternate arrange- 

 ment can include two jars of xylene in the "down series" {left to 

 right) when many slides are being stained. An absolute alcohol- 

 xylene in "up series" {right to left) is optional. 



5. Lugol's solution (page 410) 3 minutes. 



6. running water 3 minutes. 



7. 5% sodium thiosulfate, Nao 



SoOs 2-3 minutes. 



8. running water 3-5 minutes. 



9. hematoxylin, Delafields's 2-5 minutes, check after 1 minute 

 for stain intensity. Fresh solutions stain faster than old ones. If 

 not dark enough, return slides to stain. Rinse off stain in tap wa- 

 ter before checking under microscope. If slide becomes too dark, 

 convert to regressive type of stain, page 000, 



10. running water 3-5 minutes. 



11. Scott's solution (page 412) . 3 minutes. 



12. running water . 3-5 minutes. 



13. coimterstain 1 or, more minutes, depending on 



stain used. : 



Transfer slides through "vip" series, termed running up slides (or 

 dehydration) using a series of alcohols of increasing strength. Steps 

 14 and 15 can control intensity of counterstain. Carefully watch tim- 

 ing in these solutions. (From right to left, bottom row. Fig. 26) 



14. 70% alcohol 1 or more dips. 



15. 95% alcohol few dips. 



16. absolute alcohol 3 minutes or longer. 



17. absolute alcohol 2-3 minutes or longer. 



18. absolute alcohol-xylene (1:1) 



optional 2-3 minutes or longer. 



