Hematoxylin Slaining Procedures 139 



made in a few minutes and ready for immediate use; also an iron lake 

 can be prepared. No differentiation is required, and it is better than 

 hematoxylin for tissues of the central nervous system. It can replace 

 thionin for ganglia and glia cells, but not for myelin sheaths. It is good 

 for negTi bodies. The preferred fixatives are aceto-formol, Zenker's 

 (acetic or formol) or formalin — fixatives which preserve chromophilic 

 substance. 



solution: page 128, 



procedure: 



1. Deparaffinize and hydrate to water. 



2. Leave in stain overnight, or 3 hours at 56°C. 



3. Wash thoroughly and proceed to counterstain. 



4. Dehydrate, clear, and mount. 



results: 



nuclei — blue 



Hematein (Hemalum). (kornhauser, 1930). 



fixation: any general fixative, preferably containing HgCl2. 



solutions: 



Hemalum, page 129. 

 Counterstains, page 129. 



procedure: 



1. Deparaffinize and hydrate slides down to water, remove HgCl2. 



2. Stain hemalum, progressively: about 5 minutes. 



3. Wash in running water: 5-10 minutes. 



4. Counterstain. 



5. Dehydrate, clear, and mount. 



results: 



nuclei — blue 



other elements — color of counterstain 



Red Nuclear Staining 



Darrow Red. (powers et ai.., 1960). 

 fixation: any general fixative. 



