140 Hematoxylifi Staining (chap. 12) 



SOLUTION : 



Darrow Red: 



Darrow red ^ 50.0 mg. 



0.2M glacial acetic acid (pH2.7) 200.0 nd. 



Boil gently: 10 minutes. Cool and filter. Stable for 1 month. 



procedure: 



1. Use frozen sections in water, or deparaffinize paraffin sections and 

 hydrate to water. 



2. Stain in Darrow red: 20-30 minutes. 



3. Rinse in distilled water. 



4. Differentiate and dehydrate through 50%, 70%, and 95% alcohol, 

 not so slowly as to remove too much dye from nuclei, but just to 

 decolorize background. 



5. Complete dehydration in n-butyl alcohol; clear, and mount. 



results: 

 nuclei — red 

 other elements — depending on other stains applied 



comments: 



This is a good nuclear stain for contrast with blue cytoplasmic 

 stains — following luxol fast blue for example. It cannot be used with 

 nitrocellulose because the medium stains with Darrow red. 



Scarba Red (slidders et al., 1958) 

 fixation: any general fixative. 



solutions: 

 Scarba red: 



melted phenol (carbolic acid) 2.0 gm. 



neutral red 1.0 gm. 



Mix thoroughly, allow to cool, dissolve in: 



95% ethyl alcohol 15.0 ml. 



Add: 



2% aniline in water 85.0 ml. 



glacial acetic acid 1.0-3.0 ml. 



Mix well and filter. Keeps for at least 6 months. 



' Matheson Coleman and Bell. 



